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Abdominal Ischemia-Reperfusion Induced Cardiac Dysfunction Can Be Prevented by MitoTEMPO
Ahmet Akkoca1,2, Murat Cenk Celen2, Seckin Tuncer3
1Taskent Vocational School, Department of Occupational Health and Safety, Selcuk University, Konya, Turkey.
Background:
Cardiac dysfunction is secondary to acute mesenteric ischemia (AMI) and abdominal aortic aneurysms (AAA). The underlying cause of distant organ damage in the heart is the formation of oxidative stress caused by ischemia-reperfusion. In this study, we investigated the possible protective effects of a novel mitochondria-targeted antioxidant MitoTEMPO on contractile dysfunction and structural defects of the rat papillary muscle caused by abdominal ischemia-reperfusion (AIR).
Methods And Results:
In the experiments, adult Wistar-Albino rats were used and animals were divided randomly into 3 groups; sham-operated group (SHAM), an IR group that had aortic cross-clamping for 1 h followed by 2 h reperfusion, and a third group that received protective 0.7 mg/kg/day MitoTEMPO injection for 28-day before IR. As a result, it was observed that MitoTEMPO injection had a protective effect on the mechanical activities and structural properties of the papillary muscle impaired by AIR. Our study also showed that AIR disrupted the contractile function of the papillary muscle for each stimulation frequency and post-potentiation responses tested. This is common for each measured and calculated mechanical parameter and MitoTEMPO injection showed its protective effects.
Conclusion:
Consequently, calcium homeostasis seems to be impaired by AIR, and MitoTEMPO may exert its protective effect through energy metabolism by directly targeting the mitochondria.
Insights
MitoTEMPO, a novel antioxidant, protects heart muscle from damage caused by abdominal ischemia-reperfusion (AIR). This study shows its protective effects on contractile function and structure, suggesting mitochondrial targeting for therapeutic benefit.
Area of Science:
- Cardiovascular Research
- Mitochondrial Medicine
- Ischemia-Reperfusion Injury
Background:
- Cardiac dysfunction is a known complication of acute mesenteric ischemia (AMI) and abdominal aortic aneurysms (AAA).
- Ischemia-reperfusion (IR) injury leads to oxidative stress, causing damage to distant organs like the heart.
- Investigating protective agents against IR-induced cardiac damage is crucial.
Purpose of the Study:
- To evaluate the protective effects of MitoTEMPO, a novel mitochondria-targeted antioxidant, against abdominal ischemia-reperfusion (AIR) induced cardiac dysfunction.
- To assess the impact of MitoTEMPO on the contractile function and structural integrity of rat papillary muscle following AIR.
Main Methods:
- Adult Wistar-Albino rats were randomly assigned to three groups: sham-operated (SHAM), ischemia-reperfusion (IR) only, and IR with 28-day pretreatment of MitoTEMPO.
- The IR group underwent 1-hour aortic cross-clamping followed by 2 hours of reperfusion.
- Contractile function and structural properties of the papillary muscle were evaluated.
Main Results:
- Abdominal ischemia-reperfusion (AIR) significantly impaired the contractile function of the rat papillary muscle across various stimulation frequencies.
- MitoTEMPO pretreatment demonstrated a significant protective effect, preserving mechanical activities and structural integrity of the papillary muscle.
- AIR disrupted papillary muscle contractile function, affecting all measured mechanical parameters and post-potentiation responses, which was mitigated by MitoTEMPO.
Conclusions:
- Abdominal ischemia-reperfusion (AIR) appears to impair cardiac calcium homeostasis.
- MitoTEMPO exerts protective effects against AIR-induced cardiac damage, likely by targeting mitochondria and influencing energy metabolism.
