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Related Concept Videos

RNA Splicing01:32

RNA Splicing

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Alternative RNA Splicing02:18

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
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T Cell Activation and Clonal Selection01:22

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T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
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Updated: Nov 11, 2025

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
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Alternative Splicing of the Inhibitory Immune Checkpoint Receptor SLAMF6 Generates a Dominant Positive Form, Boosting

Emma Hajaj1,2,3, Elad Zisman1,2,3, Shay Tzaban1,2,3

  • 1Sharett Institute of Oncology, Hadassah Hebrew University Hospital, Jerusalem, Israel.

Cancer Immunology Research
|March 25, 2021
PubMed
Summary
This summary is machine-generated.

The short SLAMF6 isoform SLAMF6Δ17-65 enhances T-cell activation and anti-tumor immunity. Antisense oligonucleotides targeting SLAMF6 improved T-cell function and melanoma inhibition in mice.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Cancer Research

Background:

  • SLAMF6 is an Ig-superfamily receptor on T cells.
  • SLAMF6 has splice isoforms affecting T-cell function.

Purpose of the Study:

  • Investigate SLAMF6 splice isoforms' role in T-cell activation.
  • Explore SLAMF6 isoforms as a therapeutic target for cancer immunotherapy.

Main Methods:

  • Analyzed SLAMF6 V-domain splice isoforms in human T cells.
  • Assessed the functional impact of SLAMF6 isoforms on T-cell activation and cytotoxicity.
  • Developed and tested splice-switching antisense oligonucleotides (ASOs) in preclinical models.

Main Results:

  • Identified three SLAMF6 splice isoforms, with SLAMF6Δ17-65 exhibiting potent agonistic effects.
  • SLAMF6Δ17-65 costimulation involves SHP1, TBX21, and RUNX3, promoting a cytotoxic profile.
  • Immune checkpoint blockade treatment correlated with increased SLAMF6Δ17-65 in patients.
  • ASOs enhanced SLAMF6Δ17-65 expression in tumor-infiltrating lymphocytes, improving anti-melanoma activity in mice.

Conclusions:

  • SLAMF6 splice isoforms display opposing regulatory roles in T-cell function.
  • The SLAMF6Δ17-65 isoform holds therapeutic potential for enhancing anti-tumor immunity.
  • Targeting SLAMF6 splicing with ASOs represents a promising strategy for cancer immunotherapy.