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Related Experiment Video

Updated: Nov 11, 2025

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

Affinity-Switchable Lateral Flow Assay.

Yu-Hsuan Chen¹, Nitesh K Gupta¹, Hsiao-Jung Huang¹

¹Department of Chemistry, National Tsing Hua University, 101 Section 2, Kuang Fu Road, Hsinchu 30013, Taiwan, Republic of China.

Analytical Chemistry

|March 25, 2021

Summary

A novel affinity-switchable lateral flow assay (ASLFA) enables detection of molecules previously undetectable by standard LFAs. This new method broadens the application of rapid, on-site diagnostics for enzymes and small molecules.

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Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology

Published on: March 22, 2012

Area of Science:

Biotechnology
Analytical Chemistry
Biosensors

Background:

Lateral flow assays (LFAs) are vital for rapid, on-site diagnostics like pregnancy tests and infectious disease detection.
Current LFAs, including lateral flow immunoassays (LFIA) and nucleic acid lateral flow assays (NALFA), primarily detect proteins and nucleic acids.
Existing LFAs cannot detect enzyme activities or small molecules lacking specific binding partners.

Purpose of the Study:

To introduce a new lateral flow assay approach, termed affinity-switchable lateral flow assay (ASLFA), to overcome the limitations of current LFAs.
To enable the detection of molecules beyond proteins and nucleic acids, such as enzyme activities and small molecules.
To demonstrate the versatility and broad applicability of the ASLFA method.

Main Methods:

Developed an affinity-switchable lateral flow assay (ASLFA) based on a switchable binding principle.
Utilized an affinity-switchable biotin (ASB) probe and avidin protein for detection.
Demonstrated the ASLFA concept by detecting fluoride ions, NADH cofactor, and nitroreductase activity.

Main Results:

The ASLFA successfully detected target molecules by capturing activated ASB probes with avidin in the presence of the analyte.
A distinct test line was formed on the membrane, indicating successful detection.
The ASLFA proved effective for detecting fluoride ions, NADH, and nitroreductase activity.

Conclusions:

The developed affinity-switchable lateral flow assay (ASLFA) expands the detection capabilities of lateral flow technology.
ASLFA provides a general platform for the rapid, on-site detection of various molecules, including enzyme activities and small molecules.
This advancement broadens the scope of LFA applications in diagnostics and biochemical analysis.