Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The enteroviral protease target LSM14A operates outside of P-bodies to augment antiviral innate immunity.

bioRxiv : the preprint server for biology·2026
Same author

Mass spectrometry analysis of young and aged mice and human Alzheimer's disease with Lewy body pathology using on-slide tissue digestion.

Analytical and bioanalytical chemistry·2026
Same author

Spatial Profiling of Glycosaminoglycans (GAGomics) From Laser Microdissected Mouse Brain.

Molecular & cellular proteomics : MCP·2026
Same author

Optimization of LC-ExD methods for glycopeptides on an Omnitrap-Orbitrap platform.

Analytical and bioanalytical chemistry·2026
Same author

Updates to the Minimum Information Required for A Glycomics Experiment (MIRAGE) guidelines.

Analytical and bioanalytical chemistry·2026
Same author

Update and New Implementation of the MIRAGE Reporting Guidelines for Mass Spectrometry Experiments in Glycoscience.

Molecular & cellular proteomics : MCP·2025

Related Experiment Video

Updated: Nov 11, 2025

A Quantitative Glycomics and Proteomics Combined Purification Strategy
11:38

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

15.2K

Methods to improve quantitative glycoprotein coverage from bottom-up LC-MS data.

Deborah Chang1, Joseph Zaia1

  • 1Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts, USA.

Mass Spectrometry Reviews
|March 25, 2021
PubMed
Summary
This summary is machine-generated.

Data-independent acquisition (DIA) mass spectrometry offers improved site-specific quantitative glycoproteomics. Combining DIA with retention time modeling and ion mobility enhances glycopeptide coverage and discovery for biological insights.

Keywords:
glycoproteomicsion mobilitymass spectrometryquantificationretention time

More Related Videos

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
10:59

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

1.4K
A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues
09:16

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues

Published on: May 4, 2022

2.5K

Related Experiment Videos

Last Updated: Nov 11, 2025

A Quantitative Glycomics and Proteomics Combined Purification Strategy
11:38

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

15.2K
Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
10:59

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

1.4K
A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues
09:16

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues

Published on: May 4, 2022

2.5K

Area of Science:

  • Biochemistry and Molecular Biology
  • Analytical Chemistry
  • Proteomics

Background:

  • Site-specific, quantitative glycoproteomics analysis has advanced with mass spectrometry, but current methods face limitations.
  • Data-dependent acquisition (DDA) is popular but struggles with complete glycoform coverage.
  • Targeted acquisition offers precision but sacrifices throughput and discoverability.

Purpose of the Study:

  • To review the potential of data-independent acquisition (DIA) for comprehensive and quantitative site-specific glycoproteomics.
  • To explore enhancements for DIA workflows, including retention time modeling and ion mobility.
  • To enable deeper understanding of glycosylation's role in biological systems.

Main Methods:

  • Review of data-independent acquisition (DIA) mass spectrometry strategies.
  • Discussion of integrating retention time modeling for spectral interpretation.
  • Evaluation of ion mobility separation for precursor sampling and glycopeptide discovery.

Main Results:

  • DIA mass spectrometry shows promise for complete and quantitative site-specific glycoproteomics.
  • Retention time modeling and ion mobility can significantly increase DIA selectivity and coverage.
  • Existing instrumentation and bioinformatics support these enhanced DIA workflows.

Conclusions:

  • Enhanced DIA workflows offer a powerful approach for site-specific glycoproteomics.
  • These advancements facilitate accurate quantification and discovery of glycopeptides.
  • Improved glycoproteomics analysis will advance research on glycosylation's biological functions.