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Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

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Related Experiment Video

Updated: Nov 11, 2025

TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos

TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos

Published on: September 10, 2015

Tissue-Specific Ribosome Profiling in Drosophila.

Xun Chen¹, Dion Dickman²

¹Broad Institute of MIT and Harvard, Cambridge, MA, USA. xunchen1988@gmail.com.

Methods in Molecular Biology (Clifton, N.J.)

|March 25, 2021

Summary

This summary is machine-generated.

Researchers developed a new method to study how messenger RNA (mRNA) translation into proteins differs across specific tissues in Drosophila. This technique aids in understanding disease mechanisms and cellular responses.

Keywords:

Drosophila Genome-wide Muscle Next-generation sequencing Ribosome profiling Tissue-specific Translational regulation

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Last Updated: Nov 11, 2025

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Area of Science:

Molecular Biology
Genetics
Cell Biology

Background:

Dynamic regulation of messenger RNA (mRNA) translation into proteins is crucial for cellular function across diverse tissues.
Improper translational control is implicated in various diseases, underscoring the need to study its regulation.
Understanding tissue-specific translational differences is vital for deciphering cellular responses to changing environments.

Purpose of the Study:

To develop and present a novel method for profiling genome-wide translation in specific tissues or cell types.
To enable robust analysis of translational regulation with spatiotemporal precision in Drosophila melanogaster.

Main Methods:

Combined ribosome affinity purification with ribosome profiling.
Developed a simplified protocol for analyzing genome-wide translation.
Applied the method to specific tissues and cell types in Drosophila melanogaster.

Main Results:

Successfully established a method for robust, genome-wide translation profiling in specific Drosophila tissues.
The protocol allows for detailed analysis of translational regulation at the tissue and cell type level.
Demonstrated the utility of the method for studying differential translational control.

Conclusions:

The described method provides a powerful tool for investigating the complexities of translational regulation.
This approach is essential for understanding how differential translation impacts cellular function and disease.
Facilitates research into the heterogeneous nature of translational control across different biological contexts.