Related Experiment Video
Updated: Nov 11, 2025
03:33
Rapid Detection of Fecal Antigen of Helicobacter pylori Infection Based on Double Antibody Sandwich Detection Technology
Published on: May 23, 2025
425
Evaluating Host Responses to Helicobacter pylori Using ELISA and Western Blot.
Ciara Nulty1, Gillian Barber1, Ewelina Flis1
1School of Biochemistry & Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland.
Methods in Molecular Biology (Clifton, N.J.)
|March 25, 2021
Summary
Western blot and enzyme-linked immunosorbent assay (ELISA) are sensitive antibody techniques used to detect host protein changes after H. pylori infection. This study demonstrates their application in analyzing macrophage inflammatory responses.
Area of Science:
- Immunology and Microbiology
- Biochemistry and Molecular Biology
Background:
- Helicobacter pylori (H. pylori) infection can significantly alter host protein expression, particularly within macrophages.
- Understanding these alterations is crucial for elucidating the inflammatory mechanisms involved in H. pylori pathogenesis.
Purpose of the Study:
- To demonstrate the application of Western blot and ELISA for analyzing macrophage inflammatory responses.
- To highlight the sensitivity and specificity of these antibody-mediated techniques in the context of H. pylori infection.
Main Methods:
- Utilized Western blot for highly sensitive protein detection (picogram levels).
- Employed enzyme-linked immunosorbent assay (ELISA) for quantitative protein analysis (0.01-0.1 ng range).
- Applied both techniques to assess host protein expression changes in macrophages following H. pylori infection.
Main Results:
- Successfully applied Western blot and ELISA to detect and characterize protein alterations.
- Quantified specific protein changes indicative of macrophage inflammatory responses to H. pylori.
Conclusions:
- Western blot and ELISA are effective and sensitive tools for studying host-pathogen interactions.
- These techniques are valuable for investigating the molecular mechanisms of inflammation in H. pylori infections.
Related Concept Videos
01:28Peptic Ulcer Disease III: Clinical Manifestations and Diagnostic Studies
304
Peptic ulcer disease (PUD) presents with diverse symptoms depending on the location and severity of the ulcer. Clinical manifestations of peptic ulcer include dull pain and a burning sensation in the mid-epigastric region.
Few clinical manifestations differentiate gastric ulcers from duodenal ulcers. Distinctions in the location, timing, and pain relief are crucial for healthcare providers in differentiating between gastric and duodenal ulcers during clinical assessments.
Few clinical manifestations differentiate gastric ulcers from duodenal ulcers. Distinctions in the location, timing, and pain relief are crucial for healthcare providers in differentiating between gastric and duodenal ulcers during clinical assessments.
304
01:16Treating Helicobacter pylori in Peptic Ulcers: Antimicrobial Therapy
707
Helicobacter pylori, a resilient gram-negative bacterium, can thrive in the stomach's harsh, acidic environment. Infection with H. pylori leads to a cascade of events within the stomach lining. One of the critical disruptions caused by this bacterium is the interference with somatostatin production, a hormone responsible for regulating acid secretion. This interference tips the balance, escalating acid secretion and diminishing bicarbonate levels. This imbalance compromises the defensive...
707
01:33Enzyme-Linked Immunosorbent Assay
15.9K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...
15.9K
01:15Western Blotting
19.0K
Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
19.0K