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Manipulation of self-assembled three-dimensional architecture in reusable acoustofluidic device
Tan Dai Nguyen1, Van-Thai Tran1, Hejun Du1
1School of Mechanical and Aerospace Engineering, Nanyang Technological University, Nanyang, Singapore.
Electrophoresis
|March 25, 2021
Summary
This study introduces a novel method using surface acoustic waves to precisely control cell matrix patterns for tissue engineering. This technique enables advanced 3D cell architecture reconstruction, crucial for developing organs-on-chips and microvasculatures.
Area of Science:
- Biotechnology
- Tissue Engineering
- Microfluidics
Background:
- Cell architecture reconstruction is vital for tissue engineering.
- Surface acoustic waves (SAW) offer noninvasive, noncontact, and automated methods for cell self-assembly into 3D patterns.
- Existing SAW techniques require further refinement for precise control over 3D matrix architecture.
Purpose of the Study:
- To develop a method for manipulating the orientation and curvature of 3D cell matrix patterns.
- To create 3D longitudinal cell patterns using polydimethylsiloxane (PDMS) rods.
- To enhance the capabilities of organs-on-chips platforms through controlled cell architecture.
Main Methods:
- Redesigning the top wall of microfluidic chambers to control pattern curvature and orientation.
- Utilizing surface acoustic waves for noncontact manipulation of cell self-assembly.
- Integrating preinserted polydimethylsiloxane (PDMS) rods to guide the formation of 3D longitudinal patterns.
Main Results:
- Experimental results demonstrated successful manipulation of 3D matrix pattern orientation and curvature.
- The developed technique effectively created 3D longitudinal patterns along PDMS rods.
- Observed results showed good agreement with theoretical model predictions.
Conclusions:
- This research presents a significant advancement in controlling cell architecture for tissue engineering applications.
- The developed method offers a pathway to improved organs-on-chips platforms with precise cell density and architecture control.
- The 3D longitudinal patterns are well-suited for the self-assembly of microvasculatures, advancing regenerative medicine.
