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lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
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During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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Updated: Nov 11, 2025

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
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Long non-coding RNA LNC_000641 regulates pseudorabies virus replication.

Linlin Fang1, Yanni Gao1,2, Xing Liu1,2

  • 1Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.

Veterinary Research
|March 26, 2021
PubMed
Summary

Long non-coding RNAs (lncRNAs) accelerate pseudorabies virus (PRV) replication by affecting the JAK-STAT1 pathway. This study identifies lnc641 as a potential host factor for developing novel antiviral therapies against PRV infection.

Keywords:
Anti-viral activityIFN-alphaJAK/STAT1Long non-coding RNAs (lncRNAs)Pseudorabies virus

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Area of Science:

  • Molecular Biology
  • Virology
  • Genomics

Background:

  • Long non-coding RNAs (lncRNAs) are crucial gene regulators.
  • The role of lncRNAs in pseudorabies virus (PRV) infection is largely unexplored.
  • Understanding host-pathogen interactions at the lncRNA level is vital for antiviral development.

Purpose of the Study:

  • To identify lncRNAs involved in PRV infection.
  • To elucidate the function of specific lncRNAs in PRV replication.
  • To explore potential therapeutic targets for PRV.

Main Methods:

  • RNA sequencing to identify differentially expressed lncRNAs in PRV-infected cells.
  • Quantitative real-time PCR (qRT-PCR) to validate lncRNA expression.
  • Gene silencing and overexpression techniques to assess lncRNA function.
  • JAK-STAT1 pathway analysis.

Main Results:

  • 225 differentially expressed lncRNAs were identified in PRV-infected 3D4/21 cells.
  • Five upregulated lncRNAs were validated across different PRV strains.
  • lnc641 was confirmed to accelerate PRV replication.
  • lnc641 was found to inhibit the JAK-STAT1 signaling pathway.

Conclusions:

  • lnc641 is a novel host factor that promotes PRV replication by suppressing the JAK-STAT1 pathway.
  • lnc641 represents a potential therapeutic target for developing new antiviral strategies against PRV.