Can hairspray be used as a smear fixative? A comparison between two types of coating fixatives

Pairs of smears from 25 consecutive women undergoing routine screening and 30 consecutive colposcopy patients suspected of having cervical intraepithelial neoplasia were randomized and fixed with either Merckofix or a hairspray to compare their use as coating fixatives. Vacuolated cells from the cervical transformation zone were present in 9 of 25 and 24 of 30 smears fixed with hairspray against 2 of 25 and 2 of 30 of smears fixed with Merckofix in the two series, respectively. These findings indicate that the fixative effect of commercial hairsprays always should be tested before such hairsprays are introduced into routine procedures.