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[Arenavirus antigen persistence in continuous cell lines. Attempts at isolating the infectious virus]
Voprosy Virusologii
|September 1, 1977
Summary
Researchers failed to isolate infectious arenavirus from FL cells using various activation methods. Temperature changes affected antigen presence, suggesting potential proviral DNA integration into the cell genome.
Area of Science:
- Virology
- Cell Biology
- Molecular Biology
Background:
- Persistent arenavirus antigen was detected in FL cells.
- Previous attempts to isolate infectious virus were unsuccessful.
Purpose of the Study:
- To activate and isolate infectious arenavirus from FL cells.
- To investigate the persistence of arenavirus antigen in FL cells.
Main Methods:
- Tested inhibitors of macromolecular synthesis.
- Used ultraviolet irradiation and cell fusion techniques.
- Performed DNA transfection experiments.
- Cultivated FL cells at different temperatures (31°C and 41°C).
Main Results:
- All attempts to activate infectious virus yielded negative results.
- Cultivation at 31°C increased antigen-containing cells; 41°C eliminated antigen.
- No infectious virus was isolated.
Conclusions:
- Standard methods failed to rescue infectious arenavirus.
- Temperature-dependent modulation of antigen suggests complex viral-cellular interactions.
- Potential integration of proviral fragments into the host cell genome is considered.