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Related Experiment Videos

Rapid method for measuring intracellular pH in vivo.

S K Calderwood, J A Dickson

    Cell Biology International Reports
    |July 1, 1978
    PubMed
    Summary

    This study measured intracellular pH (pHi) in rat tissues using a novel triple isotope technique. Results showed normal tissues near pH 7 and demonstrated pHi

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    Area of Science:

    • Physiology
    • Biochemistry
    • Oncology

    Background:

    • Accurate measurement of intracellular pH (pHi) is crucial for understanding cellular function and disease.
    • Previous methods for pHi determination in vivo had limitations in speed and accuracy.

    Purpose of the Study:

    • To develop and validate a rapid triple isotope technique for simultaneous pHi measurement in multiple rat tissues.
    • To investigate the relationship between intracellular pH and extracellular pH (pHe) in normal and malignant tissues.
    • To assess the impact of acid-base challenges on organ pHi.

    Main Methods:

    • Utilized a triple isotope technique employing 5,5-dimethyl-2,4-oxazolidinedione-2-14C (DMO), tritiated water, and sodium chloride-36.
    • Simultaneously measured pHi in six normal rat tissues and one malignant tumor.
    • Validated results against direct capillary glass electrode pH measurements and existing literature values.

    Main Results:

    • The triple isotope technique provided results comparable to direct pH measurements.
    • Mean pHi of normal rat tissues was approximately pH 7.
    • A linear relationship was observed between pHi and pHe (range 6.9-7.6) in all organs, and organ pHi responded to NH4Cl or NaHCO3 administration.

    Conclusions:

    • The rapid triple isotope technique is a reliable method for in vivo pHi measurement in multiple tissues.
    • Normal rat tissues maintain a pHi close to 7, with a direct correlation to extracellular pH.
    • Organ pHi is responsive to systemic acid-base disturbances, highlighting its dynamic regulation.

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