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Related Experiment Video

Updated: Nov 11, 2025

Swabbing the Urban Environment - A Pipeline for Sampling and Detection of SARS-CoV-2 From Environmental Reservoirs
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Detection methods for SARS-CoV-2 in tissue.

Saskia von Stillfried1, Peter Boor2,3

  • 1Institute of Pathology, University Hospital of RWTH Aachen, Aachen, Germany.

Der Pathologe
|March 26, 2021
PubMed
Summary
This summary is machine-generated.

Detecting SARS-CoV-2 in tissues is crucial for understanding COVID-19. RNA detection methods, like RT-PCR, offer superior sensitivity and specificity compared to antigen or protein detection in tissue samples.

Keywords:
COVID-19Electron microscopyFluorescence in situ hybridizationPreanalytical phaseReverse transcriptase polymerase chain reaction

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Area of Science:

  • Pathology
  • Infectious Diseases
  • Molecular Biology

Background:

  • SARS-CoV-2 tissue analysis aids understanding of COVID-19 pathophysiology.
  • It is vital for interpreting histopathological findings and assessing occupational hazards.

Purpose of the Study:

  • To review and evaluate SARS-CoV-2 detection methods in human tissues.
  • To identify sources of error, assess specificity and sensitivity of various techniques.

Main Methods:

  • Systematic literature review of 136 studies from LitCovid database.
  • Evaluation of RNA, protein/antigen, and electron microscopy detection methods.
  • Incorporation of authors' practical experience with SARS-CoV-2 tissue analysis.

Main Results:

  • No specific histomorphological features for COVID-19 are currently established.
  • Tissue quality and preanalytical variables (e.g., post-mortem interval) are significant error sources.
  • RNA detection (RT-PCR) demonstrates higher sensitivity and specificity than antigen/protein detection; electron microscopy is time-consuming.

Conclusions:

  • Various methods exist for SARS-CoV-2 tissue detection, with RT-PCR being the preferred choice.
  • Extensive validation and harmonization of detection methods are essential for reliable results.