Effect of Fe³⁺ on the sludge properties and microbial community structure in a lab-scale A²O process

During biological wastewater treatment, ferric salt (Fe³⁺) usually serves as an inorganic flocculant to improve the agglomeration and sedimentation of suspended solids, and thus the removal efficiency of pollutants to meet the increasing strictly regulated wastewater discharge standards. In this study, we investigated the effects of Fe³⁺ on the removal efficiencies of pollutants, sludge properties, dominant flora and metabolic pathways of bacterial community in a classical anaerobic-anoxic-oxic (A²O) process. The results showed that a Fe³⁺ concentration lower than 10 mg·L^-1 could improve the removal efficiencies of chemical oxygen demand (COD) and total nitrogen (TN), while an inhibition effect was exerted at concentration higher than 10 mg·L^-1. The maximum removal efficiencies of COD and TN were 97% and 89%, respectively, under the critical Fe³⁺ concentration of 10 mg·L^-1. Total phosphorous (TP) removal was constantly positively correlated with Fe³⁺ concentration, due to the enhanced adsorption of phosphorus on activated sludge with the increase of surface roughness. Thauera displayed the highest relative abundance, and certain bacteria in Proteobacteria, Dehloromonas and Candidatus-Competibacter exhibited good adaptability to high concentration of Fe³⁺. In the context of metabolic collaterals, the most abundant functional gene families were identified to be Carbohydrate Metabolism, Amino Acid Metabolism, Cell Motility, Membrane Transport, and Replication and Repair. This study provides an extensive mechanistic insight into the impact of Fe³⁺ on the A²O process, which is of fundamental significance to exploit the contributions of inorganic salts to biological wastewater treatment.