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Related Experiment Videos

Standard operation procedure for switchSENSE DRX systems.

Hanna Müller-Landau1, Paloma Fernández Varela2

  • 1Dynamic Biosensors GmbH, Munich, Germany.

European Biophysics Journal : EBJ
|March 27, 2021
PubMed
Summary

Choosing the right molecular interaction technology can be challenging. This guide presents a standard operating procedure for switchSENSE, a microfluidic method offering multi-parameter characterization of binding kinetics and affinity.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Numerous technologies exist for studying molecular interactions in vitro.
  • Selecting the optimal method requires understanding the advantages and disadvantages of each approach, such as microfluidic surface-bound (e.g., SPR, switchSENSE) versus in-solution (e.g., ITC, MST) techniques.
  • Surface-based methods offer advantages in sample requirement and off-rate characterization, while in-solution methods simplify sample preparation and handling.

Purpose of the Study:

  • To provide a comprehensive standard operating procedure (SOP) for the switchSENSE method.
  • To guide new users through sample preparation, instrument and biochip handling, and data acquisition and analysis.
  • To assist researchers in determining the suitability of switchSENSE for their specific applications and maximize data output.
Keywords:
Biomolecular interactionsBiophysical analysisDNA-encoded addressingSOPSurface biosensorsSwitchSENSE

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Main Methods:

  • Detailed description of the switchSENSE technology, a microfluidic approach utilizing a controlled DNA-based ligand surface.
  • Explanation of how to distribute up to two different ligand molecules on the surface at a customized density and ratio.
  • Outline of the measurement process, including sample preparation, instrument operation, and data analysis.

Main Results:

  • The SOP facilitates decision-making regarding the choice of molecular interaction analysis technology.
  • Novice users can effectively acquire and analyze data using the switchSENSE method.
  • switchSENSE enables multi-parameter characterization, including binding kinetics, affinity, enzymatic activity, and conformational changes.

Conclusions:

  • The presented SOP serves as a valuable resource for researchers utilizing or considering the switchSENSE technology.
  • switchSENSE offers unique advantages for studying molecular interactions with precise control over ligand presentation.
  • The technology provides comprehensive insights into molecular binding events and functional assays.