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Related Experiment Video

Updated: Nov 11, 2025

Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry

Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry

Published on: July 17, 2017

Cellular Autofluorescence in Mouse Embryonic Fibroblasts Interferes with Antigen Detection Using Flow Cytometry.

Selcen Çelik-Uzuner^1,2, Chris O'Neill³

¹Human Reproduction and Development Unit, Kolling Institute for Medical Research, Sydney Medical School, University of Sydney, Sydney, 2065, Australia. selcen.celik@ktu.edu.tr.

Journal of Fluorescence

|March 27, 2021

Summary

This summary is machine-generated.

Cellular autofluorescence in mouse embryonic fibroblasts can interfere with immunostaining analysis. This study highlights the need for rigorous controls to ensure accurate quantitative results in flow cytometry and fluorescence microscopy.

Keywords:

Autofluorescence DNA methylation Flow cytometry Immunostaining

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Related Experiment Videos

Last Updated: Nov 11, 2025

Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry

Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry

Published on: July 17, 2017

Flow Cytometric Characterization of Murine B Cell Development

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Published on: January 22, 2021

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Published on: May 5, 2017

Area of Science:

Cell Biology
Immunology
Biotechnology

Background:

Immunostaining is a key method for analyzing cellular markers.
Flow cytometry offers quantitative analysis of large cell populations.
Standard protocols involve fixation, permeabilization, blocking, and antibody staining.

Purpose of the Study:

To investigate cellular autofluorescence in mouse embryonic fibroblasts.
To assess the impact of autofluorescence on immunostaining.
To emphasize the importance of controls in quantitative analysis.

Main Methods:

Immunostaining of mouse embryonic fibroblasts.
Analysis using flow cytometry.
Evaluation of autofluorescence across multiple fluorescence channels.

Main Results:

High levels of cellular autofluorescence were detected in mouse embryonic fibroblasts.
Autofluorescence interfered with the detection of cellular antigens using common fluorophores.
Useful analysis was restricted to a single fluorescence channel due to autofluorescence.

Conclusions:

Cellular autofluorescence presents a significant limitation for immunostaining techniques.
Thorough controls are essential for specific and quantitative analysis.
Researchers must be aware of potential autofluorescence interference in their experiments.