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Updated: Aug 19, 2026

Isolation of Sertoli Cells and Peritubular Cells from Rat Testes
Published on: February 8, 2016
[Effect of the fixation of biopsy specimens from rat testicles on the results of morphometric studies]
Abstract:
The kind of the fixation method of bioptic preparations of the testicles has a decisive influence on the morphometrically determinable average diameter of the seminiferous tubules. Under the same conditions experiments 207 preparations of the parenchyma of the testicles of rats were fixed in Bouin's and Carnoy's solution as well as in 10% formalin solution. The measuring values of the diameter of the tubules of the preparations fixed in Bouin's solution were largest. Therefore the authors recommend to perform the fixation of the bioptic preparations of the testicles in Bouin's solution. Thus standardized experimental conditions and possibilities of the comparison of morphometric findings may be got.

