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Synapsin Condensates Recruit alpha-Synuclein.

Christian Hoffmann1, Roberto Sansevrino1, Giuseppe Morabito1

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|March 28, 2021
PubMed
Summary
This summary is machine-generated.

Alpha-synuclein interacts with synapsin condensates, influencing synaptic vesicle (SV) clustering. The ratio of synapsin to alpha-synuclein is critical for forming functional SV condensates, impacting nerve terminal function.

Keywords:
liquid-liquid phase separationsynapsin 1synaptic vesiclessynucleinopathiesα-synuclein

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Biochemistry

Background:

  • Synaptic vesicle (SV) clustering is crucial for neurotransmission.
  • Synapsin 1 forms liquid-like condensates with SVs.
  • Alpha-synuclein's role in synaptic physiology is not fully understood.

Purpose of the Study:

  • To investigate the effect of alpha-synuclein on synapsin-mediated SV condensation.
  • To elucidate the role of alpha-synuclein in the regulation of synaptic vesicle clustering.

Main Methods:

  • Reconstitution approach using purified SVs from rat brains.
  • Heterologous cell system to generate synapsin condensates.
  • Analysis of synapsin and alpha-synuclein interactions within condensates.

Main Results:

  • Synapsin condensates recruit alpha-synuclein, maintaining its high mobility.
  • Synaptic vesicles enhance the rate of synapsin/alpha-synuclein condensation.
  • Alpha-synuclein alone does not cluster SVs; excess disrupts condensate formation kinetics.

Conclusions:

  • Synaptic vesicles act as catalysts for synapsin condensate formation.
  • The molar ratio of synapsin to alpha-synuclein is critical for functional condensate assembly.
  • Understanding these interactions is key to deciphering synucleinopathy pathogenesis.