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Improved sodium hydroxide digestion method without homogenization for extraction of gentamicin from renal tissue
S A Brown1, K Sugimoto, G G Smith
1Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A & M University, College Station 77843.
Antimicrobial Agents and Chemotherapy
|April 1, 1988
Abstract:
Recovery of gentamicin from renal cortical tissue (assayed by fluorescence polarization immunoassay) was highest (P less than 0.05) when using a sodium hydroxide (NaOH) digestion procedure without homogenization (90.0 +/- 5.4%), followed by homogenization with NaOH digestion (85.8 +/- 7.7%) and homogenization with trichloroacetic acid precipitation (84.4 +/- 3.3%). Simple homogenization recovered the least gentamicin (59.0 +/- 5.2%; P less than 0.05).