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iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

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Related Experiment Video

Updated: Nov 11, 2025

Human Microglia-like Cells: Differentiation from Induced Pluripotent Stem Cells and In Vitro Live-cell Phagocytosis Assay using Human Synaptosomes

Human Microglia-like Cells: Differentiation from Induced Pluripotent Stem Cells and In Vitro Live-cell Phagocytosis Assay using Human Synaptosomes

Published on: August 18, 2022

Human Pluripotent Stem Cell Differentiation to Microglia.

Laraib Ijaz¹, Madhura Nijsure¹, Valentina Fossati²

¹The New York Stem Cell Foundation Research Institute, New York, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)

|March 28, 2021

Summary

Researchers developed a new protocol using induced pluripotent stem cells (iPSCs) to create patient-specific microglia progenitors and microglia-like cells for studying central nervous system (CNS) diseases.

Keywords:

Activated microglia Differentiation Human induced pluripotent stem cells Human microglia Microglia progenitors

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Derivation of a Human Brain Organoid with Microglia Development

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Last Updated: Nov 11, 2025

Human Microglia-like Cells: Differentiation from Induced Pluripotent Stem Cells and In Vitro Live-cell Phagocytosis Assay using Human Synaptosomes

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Published on: May 31, 2022

Derivation of a Human Brain Organoid with Microglia Development

Derivation of a Human Brain Organoid with Microglia Development

Published on: January 17, 2025

Area of Science:

Neuroscience
Immunology
Stem Cell Biology

Background:

Microglia are key immune cells in the central nervous system (CNS), crucial for homeostasis and implicated in various neurological disorders.
Aberrant microglial function is linked to neurodevelopmental, neuroinflammatory, and neurodegenerative diseases.
There is a significant need for advanced in vitro models to study human microglia biology.

Purpose of the Study:

To establish a protocol for generating patient-specific microglia progenitors and microglia-like cells from induced pluripotent stem cells (iPSCs).
To provide a novel in vitro model for investigating human microglia in health and disease.

Main Methods:

Utilized induced pluripotent stem cells (iPSCs) for differentiation.
Employed specific growth factors and cytokines in serum-free conditions.
Generated mesodermal progenitors that release free-floating microglia progenitors.

Main Results:

Successfully generated microglia progenitor cells within approximately 35 days.
Achieved further maturation into microglia-like cells within an additional two weeks.
Developed a protocol yielding adherent microglia-like cells for functional studies.

Conclusions:

The protocol enables the generation of patient-specific microglia progenitors and microglia-like cells from iPSCs.
These cells serve as valuable tools for co-culture systems, chimeric mouse models, and functional studies.
This advancement facilitates research into CNS homeostasis and diseases involving microglial dysfunction.