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Studies on the autoxidation of dopamine: interaction with ascorbate.

E Pileblad1, A Slivka, D Bratvold

  • 1Department of Neurology, Mount Sinai School of Medicine of the City University of New York, New York 10029.

Archives of Biochemistry and Biophysics
|June 1, 1988
PubMed
Summary

Ascorbate can act as an antioxidant or prooxidant in reactions with dopamine (DA). This dual role, influencing oxygen consumption and hydrogen peroxide production, has implications for dopamine toxicity in neurons.

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Area of Science:

  • Biochemistry
  • Neuroscience
  • Electrochemistry

Background:

  • Dopamine (DA) is a crucial neurotransmitter with complex redox properties.
  • Understanding DA's reactivity is vital for elucidating neuronal function and toxicity.

Purpose of the Study:

  • To investigate the role of ascorbate in modulating the reaction between dopamine and oxygen.
  • To quantify oxygen consumption and hydrogen peroxide production under varying conditions.

Main Methods:

  • Utilized an oxygen electrode to monitor reactions at physiological pH (7.4) and temperature (37°C).
  • Employed diethylenetriaminepentaacetic acid (DTPA) to chelate trace metals.
  • Used catalase experiments to equate oxygen consumption with hydrogen peroxide formation.
  • Analyzed reaction products using high-performance liquid chromatography with electrochemical detection.

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Main Results:

  • Ascorbate exhibited dual activity: antioxidant (suppressing O2 consumption to 6-8%) and prooxidant (amplifying O2 consumption by 640%).
  • Antioxidant effect attributed to superoxide radical scavenging.
  • Prooxidant effect linked to redox cycling of a dopamine oxidation product.
  • Identified a novel oxidation product with low potential, distinct from 6-hydroxydopamine.

Conclusions:

  • Ascorbate's interaction with dopamine is condition-dependent, influencing oxidative stress.
  • The prooxidant activity may contribute to dopamine-mediated neurotoxicity.
  • Findings offer insights into the redox chemistry of dopamine in biological systems.