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Let-7i-5p enhances cell proliferation, migration and invasion of ccRCC by targeting HABP4

Yujie Liu1, Xing Hu2, Liang Hu3

  • 1Department of Geriatric Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, China.

BMC Urology
|March 29, 2021
PubMed
Abstract

Insights

let-7i-5p promotes clear cell renal cell carcinoma (ccRCC) progression by increasing cell proliferation, migration, and invasion. This microRNA targets HABP4, offering a potential therapeutic target for ccRCC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Clear cell renal cell carcinoma (ccRCC) is a prevalent kidney cancer.
  • Understanding the molecular mechanisms driving ccRCC is crucial for effective treatment strategies.

Purpose of the Study:

  • To investigate the role and underlying mechanisms of let-7i-5p in ccRCC.
  • To identify potential therapeutic targets for ccRCC based on let-7i-5p function.

Main Methods:

  • Bioinformatic analysis of The Cancer Genome Atlas (TCGA) database for let-7i-5p expression and targets.
  • In vitro assays including proliferation, wound healing, and Transwell invasion assays.
  • Dual-luciferase reporter assays, qRT-PCR, and Western blotting to confirm let-7i-5p and HABP4 interaction.

Main Results:

  • let-7i-5p expression is elevated in ccRCC and associated with poor survival.
  • let-7i-5p inhibition suppressed, while mimics enhanced, ccRCC cell proliferation, migration, and invasion.
  • HABP4 was identified as a direct target of let-7i-5p, with its expression decreased in ccRCC and negatively correlated with let-7i-5p levels.
  • Downregulation of HABP4 promoted ccRCC cell proliferation, migration, and invasion.

Conclusions:

  • let-7i-5p acts as a tumor promoter in ccRCC by targeting HABP4.
  • The let-7i-5p/HABP4 axis plays a significant role in ccRCC pathogenesis.
  • Targeting the let-7i-5p/HABP4 pathway presents a potential therapeutic strategy for ccRCC.
Keywords:
Cell invasionCell migrationCell proliferationClear cell renal cell carcinomaHABP4Let-7i-5p

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