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Published on: March 30, 2019
MiR-628-5p Inhibits Cervical Carcinoma Proliferation and Promotes Apoptosis by Targeting VEGF
Xiaoyan Wu1, Jianzhen Lei2, Bing Zhou2
1Department of Gynecology and Obsterics, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu, China.
Background:
It has been reported that the dysregulation of microRNAs (miRNAs) is implicated in the biological processes of diverse diseases, including the tumorigenesis of human cancers. MicroRNA-628-5p (miR-628-5p) is differentially expressed and plays a critical role in several cancers, but the role of miR-628-5p in cervical cancer has not been well studied.
Methods:
The TCGA database and RT-qPCR were used to evaluate the expression profile of miR-628-5p in cervical cancer tissues. Transfection efficiency of synthetic miRNAs was detected using RT-qPCR. The biological effects of miR-628-5p on cervical cancer cells were assessed by the CCK-8 assay, flow cytometry, western blot analysis, and the tube formation assay. The expression levels of key proteins involved in cell apoptosis, the cell cycle and the PI3K pathway were analyzed by western blot analysis. Bioinformatic analysis and the luciferase reporter assay were performed to investigate the targeted relationship between miR-628-5p and vascular endothelial growth factor (VEGF).
Results:
MiR-628-5p was downregulated and negatively correlated with Ki-67 expression in cervical cancer tissues, and its low level predicted poor survival of patients. Functional assays indicated that miR-628-5p inhibited cell proliferation and promoted cell apoptosis. Mechanically, VEGF was verified to be a downstream target of miR-628-5p. Moreover, overexpression of VEGF could reverse the effects of miR-628-5p on VEGF/PI3K/AKT signaling, cell proliferation, apoptosis, the cell cycle and angiogenesis in cervical cancer.
Conclusions:
MiR-628-5p inhibited cervical cancer cell proliferation and promoted apoptosis by targeting VEGF.
Insights
MicroRNA-628-5p (miR-628-5p) is downregulated in cervical cancer, inhibiting cell proliferation and promoting apoptosis by targeting vascular endothelial growth factor (VEGF). Low miR-628-5p levels predict poor patient survival.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- MicroRNA (miRNA) dysregulation is linked to various diseases, including cancer.
- MicroRNA-628-5p (miR-628-5p) has roles in several cancers, but its function in cervical cancer is understudied.
Purpose of the Study:
- To investigate the role and mechanism of miR-628-5p in cervical cancer.
- To determine the relationship between miR-628-5p, cell behavior, and potential targets.
Main Methods:
- TCGA database and RT-qPCR for miR-628-5p expression analysis in cervical cancer.
- Cell proliferation, apoptosis, cell cycle, and angiogenesis assays to assess miR-628-5p function.
- Western blot and luciferase reporter assays to identify downstream targets and signaling pathways.
Main Results:
- miR-628-5p was downregulated in cervical cancer tissues and correlated with poor patient survival.
- miR-628-5p suppressed cervical cancer cell proliferation and induced apoptosis.
- Vascular endothelial growth factor (VEGF) was identified as a direct target of miR-628-5p, mediating its effects on cell signaling and angiogenesis.
Conclusions:
- miR-628-5p acts as a tumor suppressor in cervical cancer.
- Targeting VEGF by miR-628-5p inhibits cervical cancer progression via the VEGF/PI3K/AKT pathway.
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