Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Membrane protein structure and dynamics probed by MicroED.

Biochemical Society transactions·2026

Same author

pH-mediated activation of the lysosomal arginine sensor SLC38A9.

FEBS letters·2026

Same author

Rapid Structural Analysis of Natural Products Using MicroED.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same author

Conformational Trajectory of the Molecular Chameleon Grazoprevir From Formulation to Target-Bound.

Chemistry (Weinheim an der Bergstrasse, Germany)·2025

Same author

Discovery of Oxyacanthine Dihydrochloride Monohydrate Polymorphs from Obfuscated Samples by Microcrystal Electron Diffraction.

ChemMedChem·2025

Same author

pH-dependent regulation in SLC38A9.

bioRxiv : the preprint server for biology·2025

Search research articles

Related Experiment Video

Updated: Nov 11, 2025

$Microcrystal Electron Diffraction of Small Molecules$

Microcrystal Electron Diffraction of Small Molecules

Published on: March 15, 2021

Publisher Correction: The cryo-EM method microcrystal electron diffraction (MicroED)

Brent L Nannenga^1,2, Tamir Gonen^3,4

¹Chemical Engineering, School for Engineering of Matter, Transport, and Energy, Arizona State University, Tempe, AZ, USA. Brent.Nannenga@asu.edu.

|March 29, 2021

Summary

No abstract available in PubMed .

More Related Videos

$Microcrystallography of Protein Crystals and In Cellulo Diffraction$

Microcrystallography of Protein Crystals and In Cellulo Diffraction

Published on: July 21, 2017

A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline

A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline

Published on: June 17, 2021

Related Experiment Videos

Last Updated: Nov 11, 2025

$Microcrystal Electron Diffraction of Small Molecules$

Microcrystal Electron Diffraction of Small Molecules

Published on: March 15, 2021

$Microcrystallography of Protein Crystals and In Cellulo Diffraction$

Microcrystallography of Protein Crystals and In Cellulo Diffraction

Published on: July 21, 2017

A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline

A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline

Published on: June 17, 2021