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Updated: Oct 12, 2026

Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
Published on: April 6, 2017
Inhibition of GDP beta S of agonist-activated phospholipase C in human platelets requires cell permeabilization
1Department of Biochemistry, University of Vermont, College of Medicine, Burlington 05405.
Abstract:
The inhibition by guanosine 5'-[beta-thio]diphosphate (GDP beta S) of phospholipase C was compared in intact and saponin-permeabilized human platelets in order to assess whether effects of GDP beta S on phospholipase C activation unrelated to guanine nucleotide binding function were occurring. GDP beta S exhibited no effect on phospholipase C activity, monitored by phosphatidic acid formation, in intact platelets that were unstimulated or stimulated with 0.5 U/ml thrombin or 20 nM ONO-11113 (a stable thromboxane A2 analogue). However, GDP beta S did cause a marked decrease in the activity of phospholipase C in saponin-permeabilized platelets. Thus GDP beta S is a viable tool for studying the role of G-proteins in transducing receptor-mediated activation of phospholipase C in platelets.
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