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Microbial soluble aromatic prenyltransferases for engineered biosynthesis.

He-Ping Chen1,2, Ikuro Abe1,3

  • 1Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, 113-0033, Japan.

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Summary

Prenyltransferase (PTase) enzymes are vital for creating prenylated natural products with better bioactivity. Microbial PTases are increasingly studied for synthesizing novel prenylated compounds.

Keywords:
BiosynthesisDHN, dihydroxynaphthaleneDMAPP, dimethylallyl diphosphateDMATS, dimethylallyltryptophan synthaseDMSPP, dimethylallyl S-thiolodiphosphateEnzyme engineeringFPP, farnesyl diphosphateGFPP, geranyl farnesyl diphosphateGPP, geranyl diphosphateGSPP, geranyl S- thiolodiphosphateIPP, isopentenyl pyrophosphateMicrobial prenyltransferasePPP, phytyl pyrophosphatePTase, prenyltransferasePrenylationRiPP, ribosomally synthesized and posttranslationally modified peptideSynthetic biologyTHN, 1,3,6,8-tetrahydroxynaphthalene

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Area of Science:

  • Biochemistry and Natural Product Biosynthesis
  • Enzymology
  • Synthetic Biology

Background:

  • Prenyltransferase (PTase) enzymes catalyze the transfer of isoprene units to substrates, forming prenylated compounds.
  • Prenylation enhances the membrane affinity and bioactivity of natural products.
  • Microbial PTases have garnered significant research interest over the past two decades.

Purpose of the Study:

  • To summarize microbial soluble aromatic PTases.
  • To highlight their application in chemoenzymatic synthesis.
  • To showcase the generation of novel prenylated compounds.

Main Methods:

  • Review of recent studies on microbial PTase identification and characterization.
  • Analysis of enzyme engineering and synthetic biology approaches.
  • Compilation of examples using microbial PTases for chemoenzymatic synthesis.

Main Results:

  • Identification and characterization of various microbial PTase enzymes.
  • Demonstration of enzyme engineering and synthetic biology strategies.
  • Successful chemoenzymatic synthesis of novel prenylated compounds.

Conclusions:

  • Microbial PTases are valuable tools for natural product biosynthesis.
  • Chemoenzymatic synthesis using PTases enables the creation of diverse prenylated molecules.
  • Further exploration of microbial PTases holds promise for novel compound discovery.