Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Predictions from deep learning propose substantial protein-carbohydrate interplay.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Gangliosides in molecular interactions and cell regulation.

The Journal of biological chemistry·2026
Same author

Predictions from Deep Learning Propose Substantial Protein-Carbohydrate Interplay.

bioRxiv : the preprint server for biology·2025
Same author

Sialylated keratan sulfates on MUC5B are Siglec-8 ligands in the human esophagus.

Glycobiology·2024
Same author

Engineered cytokine/antibody fusion proteins improve IL-2 delivery to pro-inflammatory cells and promote antitumor activity.

JCI insight·2024
Same author

Tumor necrosis factor α receptor 1A transduces the inhibitory effect on axon regeneration triggered by IgG anti-ganglioside GD1a antibodies.

Biochimica et biophysica acta. Molecular basis of disease·2024

Related Experiment Video

Updated: Nov 11, 2025

Ganglioside Extraction, Purification and Profiling
10:05

Ganglioside Extraction, Purification and Profiling

Published on: March 12, 2021

4.9K

Ganglioside Extraction, Purification and Profiling.

Mitchell J Porter1, Gao-Lan Zhang1, Ronald L Schnaar2

  • 1Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine.

Journal of Visualized Experiments : Jove
|March 29, 2021
PubMed
Summary
This summary is machine-generated.

This study presents rapid, bench-level methods for extracting and purifying gangliosides, crucial glycosphingolipids found abundantly in the brain. These techniques offer accessible analytical and preparative scale access to these bioactive molecules.

More Related Videos

Mass Spectrometric Analysis of Glycosphingolipid Antigens
13:09

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Published on: April 16, 2013

16.8K
Preparation of CD4+ T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy
10:00

Preparation of CD4+ T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy

Published on: November 8, 2016

8.8K

Related Experiment Videos

Last Updated: Nov 11, 2025

Ganglioside Extraction, Purification and Profiling
10:05

Ganglioside Extraction, Purification and Profiling

Published on: March 12, 2021

4.9K
Mass Spectrometric Analysis of Glycosphingolipid Antigens
13:09

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Published on: April 16, 2013

16.8K
Preparation of CD4+ T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy
10:00

Preparation of CD4+ T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy

Published on: November 8, 2016

8.8K

Area of Science:

  • Biochemistry
  • Neuroscience
  • Cell Biology

Background:

  • Gangliosides are glycosphingolipids with one or more sialic acid residues, vital for vertebrate cell function, particularly in the brain.
  • They are located on cell membranes, influencing protein activity, cell interactions, and serving as targets for pathogens.
  • Disruptions in ganglioside biosynthesis are linked to severe congenital nervous system disorders.

Purpose of the Study:

  • To describe efficient bench-level methods for ganglioside extraction, purification, and analysis.
  • To provide protocols for both preliminary qualitative/quantitative analyses and larger-scale purification of major ganglioside species.
  • To facilitate easier access to gangliosides for research purposes.

Main Methods:

  • Development of rapid, bench-level protocols for ganglioside extraction and purification from tissues and cells.
  • Implementation of techniques for preliminary qualitative and quantitative analysis of major gangliosides.
  • Establishment of methods for larger-scale isolation and purification of specific ganglioside species, particularly from brain tissue.

Main Results:

  • Successful demonstration of methods to extract, purify, and analyze major gangliosides within hours.
  • Validation of protocols for both small-scale analytical and large-scale preparative purposes.
  • Provision of accessible techniques for researchers studying ganglioside function and disorders.

Conclusions:

  • The described methods offer efficient and accessible means for the analysis and purification of gangliosides.
  • These techniques facilitate research into the roles of gangliosides in cellular processes and neurological disorders.
  • The study enhances the ability of researchers to obtain and study these important bioactive molecules.