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Updated: Nov 11, 2025
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Detection and Isolation of Apoptotic Bodies to High Purity
Published on: August 12, 2018
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Apoptotic Bodies: Mechanism of Formation, Isolation and Functional Relevance
Jascinta P Santavanond1, Stephanie F Rutter1, Georgia K Atkin-Smith2,3
1Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, VIC, Australia.
Sub-Cellular Biochemistry
|March 29, 2021
Summary
Apoptotic cells release membrane-bound vesicles called apoptotic extracellular vesicles (ApoEVs). These apoptotic bodies (ApoBDs) play roles in cell clearance and communication, highlighting the importance of apoptotic cell disassembly.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Apoptosis culminates in the formation of membrane-bound vesicles.
- Apoptotic extracellular vesicles (ApoEVs) are generated by dying cells.
- Apoptotic bodies (ApoBDs) are a key subset of ApoEVs, formed via apoptotic cell disassembly.
Purpose of the Study:
- To characterize the properties and functions of ApoBDs.
- To elucidate the molecular mechanisms regulating apoptotic cell disassembly.
- To review methods for isolating ApoBDs.
Main Methods:
- Differential centrifugation for ApoBD isolation.
- Filtration techniques for ApoBD separation.
- Fluorescence-activated cell sorting (FACS) for ApoBD characterization.
Main Results:
- ApoBD formation is a regulated process involving membrane blebbing and fragmentation.
- ApoBDs contribute to physiological processes like apoptotic cell clearance.
- Intercellular communication is influenced by ApoBDs.
Conclusions:
- Apoptotic cell disassembly is a critical process following cell death.
- Understanding ApoBD formation and function is vital in physiological and pathological contexts.
- Further research into ApoBDs can reveal new therapeutic targets.
Keywords:
ApoptosisApoptotic bodiesApoptotic body isolationApoptotic cell clearanceExtracellular vesiclesIntercellular communicationMore Related Videos
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