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Type 2 human papillomavirus E7 attenuates E-cadherin expression in human keratinocytes
Ji Young Song1,2, Young Min Park2,3, Soon Yong Choi4
1Department of Biological Sciences and Biotechnology, College of Life Sciences and Nanotechnology, Hannam University, Daejeon, 34054, Republic of Korea.
Journal of Microbiology (Seoul, Korea)
|March 29, 2021
Summary
Human papillomavirus type 2 (HPV-2) E7 protein down-regulates E-cadherin, a key cell adhesion molecule, in skin cells. This reduction in E-cadherin expression is linked to cutaneous warts development.
Area of Science:
- Virology
- Dermatology
- Molecular Biology
Background:
- High-risk Human Papillomaviruses (HPVs) down-regulate E-cadherin for immune evasion.
- The impact of low-risk HPV types on E-cadherin function is not well understood.
Purpose of the Study:
- To investigate if HPV type 2 (HPV-2) E7 protein alters E-cadherin expression in keratinocytes.
- To determine the role of HPV-2 E7 in the pathogenesis of cutaneous warts.
Main Methods:
- Immunohistochemistry on skin samples.
- Quantitative real-time PCR, luciferase assays, Western blot, immunocytochemistry, and electron microscopy.
- Transfection of keratinocytes with HPV-2 E7 plasmid DNA or E7-specific siRNA.
Main Results:
- HPV-2 positive warts showed significantly decreased E-cadherin expression compared to normal skin.
- E7 transfection reduced E-cadherin mRNA and protein levels, which was reversed by E7-specific siRNA.
- E7-expressing cell lines exhibited reduced E-cadherin and inhibited cell-to-cell aggregation.
Conclusions:
- HPV-2 E7 protein mediates the transcriptional repression of E-cadherin.
- Down-regulation of E-cadherin by HPV-2 E7 may contribute to the development of cutaneous warts.
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