Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

1.8K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and signal-to-noise ratio for the analyte. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.
Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called collision-induced...
1.8K
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

7.4K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
7.4K
Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

2.2K
An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a low-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.
To...
2.2K
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

6.0K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
6.0K
Mass Spectrometers01:16

Mass Spectrometers

7.6K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
7.6K
Mass Spectrometry: Molecular Fragmentation Overview01:20

Mass Spectrometry: Molecular Fragmentation Overview

4.6K
The ionization of a molecule into a molecular ion inside the mass spectrometer causes instability in the molecule's structure due to the loss of an electron. This eventually leads to the fragmentation or breaking of some bonds in the molecule. The fragmentation occurs predominantly at specific bonds to yield relatively stable fragments.
One type of fragmentation pattern is the cleavage of a single bond in the molecular ion. The cleavage leads to a radical and a cation. The cleavage can occur at...
4.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrated top-down and bottom-up proteomics enables precise characterization of proteoforms within the protein corona.

Nature communications·2026
Same author

Autophagy-Enhanced Nanoplatforms Eliminate Intracellular Bacteria and Block Bacteria-Driven Epithelial-Mesenchymal Transition for Tumor Metastasis Inhibition.

ACS nano·2026
Same author

Beta cell microRNAs function as molecular hubs of type 1 diabetes pathogenesis and as biomarkers of diabetes risk.

Diabetologia·2026
Same author

Structural insights into target detection by the <i>S. marcescens</i> type III CRISPR complex and its deployment in SNP identification.

bioRxiv : the preprint server for biology·2026
Same author

Uncovering zebrafish embryonic proteome dynamics across 16 time points during the first 24 hours of development.

bioRxiv : the preprint server for biology·2026
Same author

Top-Down Mass Spectrometry-Based Structural and Functional Proteomics: A Perspective.

Journal of mass spectrometry : JMS·2026
Same journal

Characterization of Alkane Oxidation Products in a Corona-Discharge Reactor Using Ammonia-Doped Ion Mobility-Mass Spectrometry.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Integration of a Modified Synchrotron Radiation Photoionization Time-of-Flight Mass Spectrometer with a Residual Gas Analyzer for Complementary Detection of Catalytic Products with Different Ionization Energies.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Screen for Tissue-Specific Markers of Drug-Induced Phospholipidosis Using Mass Spectrometry Imaging.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Molecular Framework Modification in Mass Spectrometry: Atom Exchange, Insertion, and Deletion.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Interlaboratory Comparison of Orbitrap Analyzers in OrbiSIMS: Noise Parameters, Intensity Scaling, and Detection Limits.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Isotope-Coded Derivatization Enables Accurate LC-MS/MS Quantification of Urinary THC-COOH without Isotope-Labeled Cannabinoid Standards.

Journal of the American Society for Mass Spectrometry·2026
See all related articles

Related Experiment Video

Updated: Nov 11, 2025

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
07:01

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

385

TopMSV: A Web-Based Tool for Top-Down Mass Spectrometry Data Visualization.

In Kwon Choi1, Tianze Jiang1, Sreekanth Reddy Kankara1

  • 1Department of BioHealth Informatics, Indiana University-Purdue University Indianapolis, Indianapolis, Indiana 46202, United States.

Journal of the American Society for Mass Spectrometry
|March 29, 2021
PubMed
Summary
This summary is machine-generated.

Top-down mass spectrometry (MS) analysis is enhanced by TopMSV, a new web-based tool. This software aids in visualizing and processing complex proteoform data, improving identification and characterization.

Keywords:
data visualizationsoftware toolstop-down mass spectrometry

More Related Videos

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

9.8K
In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS
09:48

In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS

Published on: February 15, 2016

8.6K

Related Experiment Videos

Last Updated: Nov 11, 2025

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
07:01

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

385
Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

9.8K
In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS
09:48

In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS

Published on: February 15, 2016

8.6K

Area of Science:

  • Biochemistry and Molecular Biology
  • Analytical Chemistry
  • Bioinformatics

Background:

  • Top-down mass spectrometry (MS) is crucial for analyzing intact proteoforms.
  • Manual data inspection is often required for accurate proteoform identification and characterization in top-down MS.
  • Existing MS visualization tools lack specific web-based solutions for top-down approaches.

Purpose of the Study:

  • To introduce TopMSV, a novel web-based software for top-down MS data processing and visualization.
  • To provide researchers with an accessible platform for interactive analysis of top-down MS data.
  • To integrate spectral deconvolution and proteoform identification tools within a unified visualization framework.

Main Methods:

  • Development of a web-based application, TopMSV.
  • Integration of spectral deconvolution algorithms.
  • Incorporation of proteoform identification software.
  • Implementation of interactive data visualization features within a web browser.

Main Results:

  • TopMSV offers interactive visualization of top-down MS data.
  • The tool facilitates the annotation of MS data using integrated analysis results.
  • It streamlines the process of proteoform identification and characterization.

Conclusions:

  • TopMSV addresses the need for user-friendly, web-based visualization tools in top-down MS.
  • The integrated approach enhances the efficiency and accuracy of proteoform analysis.
  • This tool empowers researchers to better interpret complex top-down mass spectrometry data.