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Updated: Nov 11, 2025
Stimulation of Notch Signaling in Mouse Osteoclast Precursors
Published on: February 28, 2017
The role of Notch ligand Jagged1 in osteosarcoma proliferation, metastasis, and recurrence
Jianping Zhang1, Na Li2, Siyu Lu1
1Department of Orthopedic Surgery, 920th Hospital of Joint Logistics Support Force, Daguan Road 212#, Kunming, 650032, China.
Background:
Osteosarcoma is the most common primary bone cancer occurring in young adults and the 5-year survival rate of patients with metastatic osteosarcoma is less than 30% due to high metastatic recurrence and drug resistance. Notch is a highly conserved cell to cell signaling pathway in evolution, and Jagged1 is an important ligand of Notch. Although some studies have found that Notch receptors and ligands including Jagged1 were highly expressed in osteosarcoma tissues and osteosarcoma cells, the role of Jagged1 in osteosarcoma progression and metastasis are still not clear.
Methods:
Tumor tissues were collected from 68 patients and immunohistochemical staining was employed to group these patients by expression of Jagged1. Real-time quantitative PCR and Western blotting were used to detect the expression of Jagged1. We used siRNA to knockdown the expression of Jagged1 in F5M2 cells. Colony formation assay and MTT were employed to detect and analyze the proliferation of F5M2 cells with or without knockdown of Jagged1. Transwell assay were used to detect the migration and invasion of F5M2 cells.
Results:
In this study, we found that the high expression of Jagged1 is closely related to the metastasis and recurrence of osteosarcoma in 68 clinical specimens. The expression of Jagged1 in F5M2 cells with high metastasis was significantly higher than that in F4 cells with low metastasis. Knockdown of Jagged1 led to lower ability of proliferation, migration, and invasion in F5M2 cells.
Conclusion:
The high expression of Jagged1 is closely related to the metastasis and recurrence of osteosarcoma. Knockdown of Jagged1 significantly reduced the proliferation, migration, and invasion of osteosarcoma cells. Our results suggested that knockdown of Jagged1 may be a potentially effective treatment for metastatic osteosarcoma.
Insights
High Jagged1 expression correlates with osteosarcoma metastasis and recurrence. Reducing Jagged1 (Notch ligand) inhibits cancer cell proliferation, migration, and invasion, suggesting a potential treatment target.
Area of Science:
- Oncology
- Molecular Biology
- Cell Signaling
Background:
- Osteosarcoma, a common bone cancer in young adults, has poor survival rates due to metastasis and drug resistance.
- The Notch signaling pathway, involving ligands like Jagged1, is implicated in various cancers.
- The specific role of Jagged1 in osteosarcoma progression and metastasis remains unclear.
Purpose of the Study:
- To investigate the association between Jagged1 expression and osteosarcoma metastasis.
- To determine the functional role of Jagged1 in osteosarcoma cell proliferation, migration, and invasion.
Main Methods:
- Immunohistochemical staining of 68 clinical osteosarcoma specimens to assess Jagged1 expression.
- Real-time quantitative PCR and Western blotting to quantify Jagged1 levels.
- siRNA-mediated knockdown of Jagged1 in osteosarcoma cell lines (F5M2) followed by proliferation (colony formation, MTT assays) and migration/invasion (Transwell assays) assessments.
Main Results:
- High Jagged1 expression in clinical specimens significantly correlated with osteosarcoma metastasis and recurrence.
- Jagged1 expression was higher in highly metastatic F5M2 cells compared to low-metastatic F4 cells.
- Knockdown of Jagged1 markedly reduced the proliferation, migration, and invasion capabilities of osteosarcoma cells.
Conclusions:
- Elevated Jagged1 expression is a significant indicator of osteosarcoma metastasis and recurrence.
- Targeting Jagged1 through knockdown effectively suppresses osteosarcoma cell growth and spread.
- Inhibition of Jagged1 presents a promising therapeutic strategy for managing metastatic osteosarcoma.
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