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Related Concept Videos

CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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What is Genetic Engineering?00:49

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CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
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[Progress in gene editing technologies for Saccharomyces cerevisiae].

Hongbiao Li1, Xiaolin Liang1,2, Jingwen Zhou1,2

  • 1National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, Jiangsu, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|March 30, 2021
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Summary

This review summarizes gene editing technologies for Saccharomyces cerevisiae, highlighting advanced systems like CRISPR/Cas that are replacing older methods for engineering yeast cell factories.

Keywords:
CRISPRSaccharomyces cerevisiaegene editingmulti-copy integration

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Area of Science:

  • Biotechnology
  • Synthetic Biology
  • Microbial Engineering

Background:

  • Saccharomyces cerevisiae is a key host in metabolic engineering.
  • Advanced gene editing is crucial for constructing S. cerevisiae cell factories.
  • Newer gene editing systems are supplanting older homologous recombination methods.

Purpose of the Study:

  • To review the principles and applications of gene editing in S. cerevisiae.
  • To detail classical and modern gene editing techniques.
  • To discuss the future prospects of gene editing in yeast.

Main Methods:

  • Overview of classical gene editing techniques.
  • Elaboration of endonuclease-based systems: MegNs, ZFNs, and TALENs.
  • Discussion of recent advancements including CRISPR/Cas, multi-copy integration, and genome-scale editing.

Main Results:

  • Classical gene editing methods are being phased out.
  • Endonuclease-based systems offer precise genome modification.
  • CRISPR/Cas and other advanced techniques enable complex metabolic pathway engineering and large-scale genome editing.

Conclusions:

  • Gene editing technologies have significantly advanced S. cerevisiae metabolic engineering.
  • CRISPR/Cas and related systems offer powerful tools for yeast synthetic biology.
  • Future directions include further optimization and application of genome-scale editing for novel cell factory designs.