Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Replication in Eukaryotes02:31

Replication in Eukaryotes

185.1K
Overview
185.1K
Replication in Eukaryotes01:29

Replication in Eukaryotes

15.8K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
15.8K
DNA Replication02:40

DNA Replication

54.8K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
54.8K
The Replisome03:01

The Replisome

36.8K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
36.8K
The Replisome03:01

The Replisome

8.4K
8.4K
Chromosome Replication02:31

Chromosome Replication

9.7K
Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
9.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cellular replisomes are powered by flex-fuel motors for unwinding DNA.

Nature communications·2026
Same author

Structural and cellular insights into DCTPP1 antagonists and their synergistic action with DNMT inhibitors.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Supercoiled DNA recognition and cleavage control in topoisomerase VI.

Nature communications·2026
Same author

Chromatin buffers torsional stress during transcription.

Science (New York, N.Y.)·2025
Same author

Torsion is a dynamic regulator of DNA replication stalling and reactivation.

Nature communications·2025
Same author

Mechanistic insights into direct DNA and RNA strand transfer and dynamic protein exchange of SSB and RPA.

Nucleic acids research·2025
Same journal

Lactate as a Chemical Modification on Proteins and Metabolites.

Annual review of biochemistry·2026
Same journal

Nucleocytoplasmic Transport.

Annual review of biochemistry·2026
Same journal

Packaging of Single-Stranded RNA in Viruses and Virus-Like Particles.

Annual review of biochemistry·2026
Same journal

Shaping of the Infant Gut Microbiome by Milk Oligosaccharides.

Annual review of biochemistry·2026
Same journal

Proteostasis Deregulation by Metabolism Drives the Hallmarks of Cancer.

Annual review of biochemistry·2026
Same journal

JoAnne Stubbe's Radical Path: A Story of Passion, Curiosity, and Persistence.

Annual review of biochemistry·2026
See all related articles

Related Experiment Video

Updated: Nov 11, 2025

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

628

Structural Mechanisms for Replicating DNA in Eukaryotes.

Ilan Attali1, Michael R Botchan2, James M Berger1

  • 1Department of Biophysics and Biophysical Chemistry, Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA;

Annual Review of Biochemistry
|March 30, 2021
PubMed
Summary
This summary is machine-generated.

DNA replication relies on molecular machines called replisomes. This study explores the physical mechanisms behind replisome assembly and function in eukaryotic cells.

Keywords:
ATPaseDNA replicationMCMORCPCNAclamp loaderhelicasepolymeraseprimase

More Related Videos

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

3.9K
Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level
10:11

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level

Published on: July 26, 2024

1.4K

Related Experiment Videos

Last Updated: Nov 11, 2025

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

628
Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

3.9K
Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level
10:11

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level

Published on: July 26, 2024

1.4K

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Cellular Biology

Background:

  • DNA replication is essential for cell division and requires precise coordination of multiple proteins.
  • Replisomes are large, dynamic molecular machines responsible for DNA duplication.
  • Understanding replisome dynamics is crucial for comprehending genome stability.

Purpose of the Study:

  • To elucidate the physical principles governing eukaryotic replisome assembly.
  • To describe the conformational changes and compositional shifts during replisome progression.
  • To provide insights into the orchestrated function of enzymes and scaffolding factors in DNA replication.

Main Methods:

  • Review of recent advancements in structural biology and biophysical techniques.
  • Analysis of dynamic protein-nucleic acid interactions within replisomes.
  • Computational modeling of replisome dynamics and conformational states.

Main Results:

  • Detailed insights into the structural organization of eukaryotic replisomes.
  • Characterization of key conformational transitions during DNA synthesis.
  • Identification of critical interactions between replisome components.

Conclusions:

  • The physical basis of replisome construction and progression is increasingly understood.
  • Dynamic remodeling of replisome architecture facilitates efficient and accurate DNA replication.
  • Further research on replisome mechanics will advance our knowledge of genome maintenance.