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Let-7a-5p regulates the inflammatory response in chronic rhinosinusitis with nasal polyps

Jianwei Zhang1, Lei Han2, Feng Chen3

  • 1Department of Otolaryngology-Head and Neck Surgery, Shanghai Pudong New District Gongli Hospital/Second Military Medical University Affiliated Hospital, Shanghai, 200135, China.

Diagnostic Pathology
|March 31, 2021
PubMed
Abstract

Insights

Let-7a-5p is downregulated in chronic rhinosinusitis with nasal polyps (CRSwNP). This microRNA regulates inflammation via the IL-6/Ras-MAPK pathway in CRSwNP.

Area of Science:

  • Molecular Biology
  • Immunology
  • Otolaryngology

Background:

  • Let-7a-5p acts as a tumor suppressor in nasopharyngeal carcinoma.
  • The role of let-7a-5p in chronic rhinosinusitis with nasal polyps (CRSwNP) remains uninvestigated.

Purpose of the Study:

  • To investigate the expression pattern of let-7a-5p in CRSwNP.
  • To elucidate the functional role of let-7a-5p in CRSwNP pathogenesis.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) to assess let-7a-5p, TNF-α, IL-1β, and IL-6 expression.
  • Western blot to analyze Ras-MAPK pathway protein levels.
  • Dual luciferase reporter and RNA pull-down assays to confirm let-7a-5p and IL-6 interaction.

Main Results:

  • Let-7a-5p was significantly downregulated in CRSwNP tissues and cells.
  • Increased expression of TNF-α, IL-1β, and IL-6 observed in CRSwNP.
  • Let-7a-5p mimic suppressed TNF-α, IL-1β, and IL-6 expression and inhibited the Ras-MAPK pathway.
  • IL-6 identified as a direct target gene of let-7a-5p.

Conclusions:

  • Let-7a-5p plays a crucial role in regulating inflammatory responses in CRSwNP.
  • The let-7a-5p/IL-6 axis modulates the Ras-MAPK pathway in CRSwNP.
  • Findings suggest let-7a-5p as a potential therapeutic target for CRSwNP.
Keywords:
Chronic rhinosinusitis with nasal polypsIL-6Inflammatory responseLet-7a-5pRas-MAPK

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