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Urethane-induced lung carcinogenesis.

Francesca Sozio¹, Tiziana Schioppa¹, Silvano Sozzani²

¹Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy; Humanitas Clinical and Research Center-IRCCS, Rozzano-Milano, Italy.

Methods in Cell Biology

|March 31, 2021

Summary

This summary is machine-generated.

This study details a protocol for inducing lung cancer in resistant C57BL/6J mice using urethane (ethyl carbamate). Multiple injections are key to overcoming genetic resistance and achieving reproducible lung tumor formation in this valuable cancer model.

Keywords:

C57BL/6J strain Chemical carcinogenesis Flow cytometry Lung cancer Urethane

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Area of Science:

Oncology
Genetics
Carcinogenesis

Background:

Chemical carcinogenesis and genetically engineered mouse models are crucial for understanding cancer development.
Urethane (ethyl carbamate) induces Kras-driven lung cancer in mice, but susceptibility varies among strains.
C57BL/6 mice are genetically resistant to lung carcinogenesis, limiting their use in certain cancer studies.

Purpose of the Study:

To describe a protocol for effective urethane-induced lung cancer in the C57BL/6J mouse strain.
To overcome the genetic resistance of C57BL/6J mice to lung carcinogenesis.

Main Methods:

Utilizing urethane (ethyl carbamate) for chemical induction of lung tumors.
Employing a protocol of multiple urethane injections to enhance tumor induction.
Focusing on the C57BL/6J mouse strain to study lung cancer mechanisms.

Main Results:

A reproducible method for inducing lung tumors in C57BL/6J mice was established.
Multiple urethane injections were found to be necessary to overcome genetic resistance.
The protocol facilitates the study of Kras-driven lung cancer in a commonly used mouse strain.

Conclusions:

This protocol enables effective lung tumor induction in genetically resistant C57BL/6J mice.
The findings support the use of C57BL/6J mice in urethane-induced lung cancer models.
This approach aids in investigating genotype and environmental factors in lung cancer development.