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CRISPR based development of RNA editing and the diagnostic platform
Lakkakula Satish1, Gunamalai Lavanya2, Thirupathi Kasthuri3
1Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer Sheva, Israel; The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Bergman Campus, Beer Sheva, Israel.
Progress in Molecular Biology and Translational Science
|March 31, 2021
Summary
The Clustered Regularly Interspersed Short Palindromic Repeat-CRISPR-Associated (CRISPR-Cas) system enables precise gene editing and RNA modification. Advances in CRISPR-Cas9 technology are driving innovation in diagnostics and biosensors, though challenges remain for full potential.
Area of Science:
- Molecular Biology
- Biotechnology
- Genetics
Background:
- The Clustered Regularly Interspersed Short Palindromic Repeat-CRISPR-Associated (CRISPR-Cas) system has revolutionized gene editing and expression control.
- Current research focuses on nuclease-dependent genome editing at the DNA level.
- Emerging techniques leverage CRISPR-Cas for RNA sequence modifications.
Purpose of the Study:
- To review recent advancements in CRISPR-Cas9 genome editing technology.
- To explore CRISPR-based RNA editing mechanisms and diagnostic applications.
- To discuss the development of CRISPR-Cas-based biosensors.
Main Methods:
- Review of current literature on CRISPR-Cas9 gene editing.
- Analysis of molecular mechanisms for CRISPR-based RNA editing.
- Examination of diagnostic tools and biosensor applications.
Main Results:
- CRISPR-Cas systems offer precise control over gene expression and genome editing.
- RNA editing using CRISPR-Cas techniques is a rapidly developing area.
- Integration with lateral flow methods enhances diagnostic capabilities, offering speed, sensitivity, and affordability.
Conclusions:
- CRISPR-Cas technology holds significant promise for biomedical research and diagnostics.
- Further technological development is necessary to overcome existing obstacles and realize the full potential of CRISPR-Cas systems.
- CRISPR-Cas-based biosensors represent a novel frontier in molecular diagnostics.
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