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W-7 at calmodulin-antagonistic concentrations facilitates noradrenaline release from rat brain cortex slices
W Reimann1, U Köllhofer, B Wagner
1Gödecke Research Institute, Department of Pharmacology, Freiburg, F.R.G.
European Journal of Pharmacology
|March 15, 1988
Abstract:
Slices from rat brain occipital cortex were preincubated with [3H]noradrenaline then superfused and stimulated electrically. W-7, 10 mumol/l, enhanced the stimulation-evoked overlow. Binding experiments with W-7 evidenced weak displacement of [3H]yohimbine and no displacement of [3H]prazosin. Release experiments with the addition of clonidine, cocaine or phentolamine provided no evidence for major interference of W-7 with alpha-adrenergic or uptake mechanisms. Facilitation could have been due to inhibition of a calmodulin-dependent enzyme which could not be identified from the present results.