Protocol for comprehensive RNA sequencing analysis of murine long non-coding RNAs during aging
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Updated: Nov 11, 2025
Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
Published on: May 18, 2017
Xinyue Lu1, Qiuzhong Zhou2, Jin Liu3
1School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China.
Abstract:
Comprehensive analyses of lncRNAs in aging have been lacking because previous studies have mainly focused on the protein-coding genes during aging. Here, we describe a protocol for the organism-wide analysis of murine lncRNAs during aging. We provide step-by-step instructions to identify lncRNAs that contribute to aging and to determine their underlying functions in each tissue. We further describe methods to compare the lncRNA expression patterns and dynamic changes among multiple tissues. For complete details on the use and execution of this protocol, please refer to Zhou et al. (2020).
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