Protocol for comprehensive RNA sequencing analysis of murine long non-coding RNAs during aging

Area of Science:

• Gerontology
• Molecular Biology
• Genomics

Background:

• Previous aging research primarily focused on protein-coding genes.
• Comprehensive analysis of long non-coding RNAs (lncRNAs) in aging is lacking.
• Understanding lncRNA roles in aging is crucial for identifying novel therapeutic targets.

Purpose of the Study:

• To present a novel protocol for organism-wide analysis of murine lncRNAs during aging.
• To identify lncRNAs implicated in the aging process.
• To elucidate the functional roles of these lncRNAs within specific tissues.

Main Methods:

• Development of a step-by-step protocol for lncRNA analysis in aging mice.
• Tissue-specific identification of aging-associated lncRNAs.
• Comparative analysis of lncRNA expression patterns and dynamics across multiple tissues.

Main Results:

• Establishment of a comprehensive method for studying lncRNAs in aging.
• Identification of key lncRNAs contributing to aging.
• Characterization of tissue-specific lncRNA functions and expression dynamics during aging.

Conclusions:

• The developed protocol enables detailed investigation of lncRNAs in aging.
• This approach facilitates the discovery of novel aging mechanisms driven by lncRNAs.
• The findings pave the way for targeted interventions in age-related diseases.