Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cleaning, Sterilization, and Disinfection01:30

Cleaning, Sterilization, and Disinfection

9.1K
Cleaning, disinfection, and sterilization are the methods that help to break the infection chain and prevent disease.
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
9.1K
Hand hygiene01:23

Hand hygiene

5.0K
Asepsis is the practice of preventing or breaking the chain of infection. The nurse employs aseptic techniques to prevent the spread of microorganisms and reduce the risk of diseases. Hand hygiene is the cornerstone of aseptic techniques and is classified into medical and surgical asepsis. Medical asepsis includes hand hygiene and the use of gloves. Surgical asepsis, or the sterile technique, refers to practices that render and keep objects and areas free of microorganisms.
Hand washing...
5.0K
Methods of Sterilization II: Chemical Methods01:30

Methods of Sterilization II: Chemical Methods

8.4K
In healthcare, the chemical method of sterilization uses chemical sterilants to treat surgical instruments and medical supplies to help prevent the transmission of infectious pathogens to patients. Due to heat sensitivity, most medical supplies and equipment should not be exposed to high temperatures. These parts include rubber, plastic, glass, and other similar elements.
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
8.4K
Bacterial Signaling01:30

Bacterial Signaling

38.3K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
38.3K
Physical Methods for Controlling Microbial Growth: Radiation and Filtration01:26

Physical Methods for Controlling Microbial Growth: Radiation and Filtration

709
Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.
709
Asepsis01:28

Asepsis

2.5K
The condition of being free from disease-causing living pathogens is asepsis. Aseptic techniques include a set of standard practices to achieve asepsis. An example is the regular environmental cleaning of all parts of the healthcare facility and hand hygiene at home before preparing or eating food. Medical and surgical asepsis in healthcare practice protects patients from harmful pathogens, minimizes the risk of contamination of susceptible sites, and reduces the risk of infection transmission.
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inhalation Exposure to 2,4-Dichlorophenoxyacetic Acid Causes Tracheal Damage-A Study in Rats.

Toxics·2026
Same author

Ki-67 staining pattern as a prognostic biomarker for advanced acral melanoma.

Anais brasileiros de dermatologia·2026
Same author

Retinoid X Receptor Alpha and Melanocytes: A Translational Perspective.

Pathology international·2026
Same author

Type I and type III collagen immunoexpression in rabbit skin biopsy samples treated with rosuvastatin gel and autologous platelet-rich plasma.

Acta cirurgica brasileira·2025
Same author

Hepatic artery aneurysm with no proximal neck and proper hepatic artery bifurcation involvement.

Jornal vascular brasileiro·2024
Same author

Chronic inhalation exposure to the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) alters cardiac collagen in Wistar rats.

Toxicology research·2024

Related Experiment Video

Updated: Nov 11, 2025

Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory
13:39

Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory

Published on: July 7, 2023

17.0K

Does washing medical devices before and after use decrease bacterial contamination?: An in vitro study.

Gisele Alborghetti Nai1,2,3,4, Denis Aloísio Lopes Medina3,4,5, Cesar Alberto Talavera Martelli2,4,5

  • 1Department of Pathology.

Medicine
|March 31, 2021
PubMed
Summary

Surface treatments on medical prostheses can reduce bacterial contamination. Antibiotic solutions were most effective, particularly against Staphylococcus epidermidis, while lidocaine also showed promise in post-contamination washing.

More Related Videos

Antimicrobial Characterization of Advanced Materials for Bioengineering Applications
08:08

Antimicrobial Characterization of Advanced Materials for Bioengineering Applications

Published on: August 4, 2018

22.5K
High-throughput Identification of Bacteria Repellent Polymers for Medical Devices
10:43

High-throughput Identification of Bacteria Repellent Polymers for Medical Devices

Published on: November 5, 2016

9.3K

Related Experiment Videos

Last Updated: Nov 11, 2025

Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory
13:39

Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory

Published on: July 7, 2023

17.0K
Antimicrobial Characterization of Advanced Materials for Bioengineering Applications
08:08

Antimicrobial Characterization of Advanced Materials for Bioengineering Applications

Published on: August 4, 2018

22.5K
High-throughput Identification of Bacteria Repellent Polymers for Medical Devices
10:43

High-throughput Identification of Bacteria Repellent Polymers for Medical Devices

Published on: November 5, 2016

9.3K

Area of Science:

  • Biomaterials Science
  • Infectious Disease Research
  • Medical Device Engineering

Background:

  • Surface treatments for medical devices aim to prevent infections and reduce device replacement.
  • Bacterial contamination of vascular prostheses (silicone and expanded polytetrafluoroethylene) is a significant clinical concern.

Purpose of the Study:

  • To evaluate the efficacy of pre- and postcontamination washing strategies in reducing bacterial contamination on different medical prostheses.
  • To assess the impact of antibiotic solutions, chlorhexidine, and lidocaine on common bacterial pathogens.

Main Methods:

  • Two types of vascular prostheses (silicone and expanded polytetrafluoroethylene) were used and divided into contaminated, pre-treated, and post-treated groups.
  • Treatments involved antibiotic solution, chlorhexidine, or lidocaine.
  • Bacterial contamination was quantified using fractal dimension analysis after incubation and culturing.

Main Results:

  • Antibiotic solution demonstrated high efficacy in inhibiting bacterial growth, especially Staphylococcus epidermidis, in both pre- and postcontamination washing.
  • Chlorhexidine reduced Staphylococcus aureus colonization by 53% in pre-washing and showed 60% reduction and absence of Enterococcus faecalis colonization in post-washing.
  • Lidocaine inhibited Staphylococcus aureus growth in postcontamination washing, suggesting its potential as an adjuvant treatment.

Conclusions:

  • Antibiotic solutions are highly effective in preventing bacterial colonization on medical prostheses.
  • Lidocaine presents a viable alternative adjuvant treatment for reducing Staphylococcus aureus contamination after device implantation.
  • Surface treatments offer a promising approach to mitigate infection risks associated with medical devices.