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Related Experiment Video

Updated: Nov 10, 2025

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

Published on: September 21, 2011

Flow management strategies for a connected purification process.

Mathias Goebel¹, Rui Rodrigues¹, Lars Pampel¹

¹Novartis Pharma AG, Basel, Switzerland.

Biotechnology and Bioengineering

|March 31, 2021

Summary

This summary is machine-generated.

This study presents four flow management strategies for connected purification processes, crucial for efficient biomanufacturing. These strategies address flow disparities in linked filtration units, optimizing downstream processing.

Keywords:

connected purification process flow management ultrafiltration

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Area of Science:

Bioprocessing and downstream purification
Chemical and process engineering
Filtration technologies

Background:

Connected downstream processing integrates multiple unit operations, eliminating intermediate storage vessels.
This approach is vital for small-scale biomanufacturing facilities.
Integrated processes necessitate sophisticated control strategies to manage inter-unit dependencies.

Purpose of the Study:

To propose and evaluate different flow management strategies for connected purification processes.
To address the challenge of flow disparities between connected unit operations, such as virus filtration and tangential flow filtration.
To enable seamless operation of multiple purification steps as a single unit.

Main Methods:

Development of four distinct flow management strategies.
Implementation and evaluation of these strategies at the bench scale.
Analysis of advantages and challenges associated with each proposed strategy.

Main Results:

Demonstration of bench-scale results for the proposed flow management strategies.
Identification of effective approaches for managing flow disparities in connected systems.
Evaluation of the performance and feasibility of each strategy.

Conclusions:

Robust flow management is essential for the successful implementation of connected downstream processes.
The proposed strategies offer solutions for maintaining process integrity and efficiency.
This work contributes to optimizing biomanufacturing workflows through advanced process control.