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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Nov 10, 2025

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Published on: August 15, 2013

High-Throughput Raman Flow Cytometry and Beyond.

Julia Gala de Pablo¹, Matthew Lindley¹, Kotaro Hiramatsu^1,2,3,4

¹Department of Chemistry, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8654, Japan.

Accounts of Chemical Research

|March 31, 2021

Summary

This summary is machine-generated.

High-throughput Raman flow cytometry uses coherent Raman scattering for direct, label-free cell analysis. This advanced technique overcomes traditional flow cytometry limitations, enabling faster and more detailed cellular insights for diverse biological applications.

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Last Updated: Nov 10, 2025

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Area of Science:

Biophysics
Analytical Chemistry
Cell Biology

Background:

Traditional flow cytometry uses fluorescent labels for cell phenotyping, which is indirect and has limitations.
Current methods are time-consuming and incompatible with cell therapy.
Raman flow cytometry offers direct, label-free chemical fingerprinting of cells.

Purpose of the Study:

To review advances, challenges, and opportunities in coherent Raman flow cytometry.
To highlight the principles and techniques of coherent Raman scattering methods.
To discuss applications and future directions in high-throughput cell analysis.

Main Methods:

Coherent Raman scattering techniques including Stimulated Raman Scattering (SRS) and Coherent Anti-Stokes Raman Scattering (CARS).
Multiplex CARS, Fourier-transform CARS, SRS imaging flow cytometry, and Raman image-activated cell sorting (RIACS).
Direct measurement of intracellular molecules for label-free single-cell metabolic phenotyping.

Main Results:

Coherent Raman scattering significantly enhances light-sample interaction for high-throughput analysis.
Enables applications in microbiology, lipid biology, cancer detection, and cell therapy.
Overcomes limitations of spontaneous Raman scattering's low throughput (1 event per second).

Conclusions:

Coherent Raman flow cytometry provides high-throughput, label-free, and nondestructive single-cell analysis.
Emerging opportunities include increased sensitivity, integration with microfluidics, machine learning, and in vivo applications.
This technology promises to revolutionize cell biology research and clinical applications.