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Creating clear and informative image-based figures for scientific publications.

Helena Jambor1, Alberto Antonietti2,3, Bradly Alicea4

  • 1Mildred Scheel Early Career Center, Medical Faculty, Technische Universität Dresden, Dresden, Germany.

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Summary
This summary is machine-generated.

Scientific figures often lack accessibility features, hindering data interpretation. This study reveals common image legibility issues in top journals, emphasizing the need for better bioimage publishing standards.

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Area of Science:

  • Life Sciences
  • Bioimage Analysis
  • Scientific Publishing

Background:

  • Figures are crucial for data presentation in scientific publications.
  • Accessibility and interpretability of scientific images are often overlooked.
  • Existing guidelines focus on image manipulation and acquisition, not reader comprehension.

Purpose of the Study:

  • To systematically assess the legibility and interpretability of non-blot images in leading scientific journals.
  • To identify common pitfalls in scientific figure design and presentation.
  • To provide recommendations for improving bioimage accessibility and quality.

Main Methods:

  • Analysis of 580 non-blot image-based papers from top 15 journals in plant sciences, cell biology, and physiology.
  • Evaluation of figures for common accessibility issues: scale bars, insets, colorblind accessibility, and clarity of labels/annotations.
  • Assessment of figure legends for sufficient explanations of depicted species, tissues, or objects.

Main Results:

  • A significant percentage of published figures contained accessibility issues.
  • Common problems included missing scale bars, poor inset design, and color choices unsuitable for colorblind readers.
  • Insufficient explanations in figure legends were frequently observed.
  • Only a small fraction of papers (2-16%) met all assessed good practice criteria for image figures.

Conclusions:

  • Current standards for bioimage publishing require improvement to ensure data accessibility.
  • Addressing common pitfalls in image design, annotation, and legend description is essential.
  • Implementing the study's recommendations can enhance the clarity and interpretability of scientific figures for a broader audience.