High efficiency in-situ biogas upgrading in a bioelectrochemical system with low energy input

Biogas produced from anaerobic digestion usually contains 30%-50% CO_2, much of which must be removed, before utilization. Bioelectrochemical biogas upgrading approaches show promise, however, they have not yet been optimized for practical applications. In this study, a bioelectrochemical system with low energy input (applied cathode potential of -0.5 V vs. standard hydrogen electrode, SHE) was used for in-situ biogas upgrading. High efficiency CO₂ conversion (318.5 mol/d/m²) was achieved when the system was operated with an organic load of 1.7 kgCOD/(m³ d). Methane content in the upgraded biogas was 97.0% and CO₂ concentrations stayed below 3%, which is comparable to biogas upgraded with more expensive and less sustainable physiochemical approaches. The high efficiency of this approach could likely be attributed to a significant enrichment of Methanothrix (92.7%) species on the cathode surface that were expressing genes involved in both acetogenic methanogenesis and direct electron transfer (DET). Electromethanogenesis by these organisms also increased proton consumption and created a higher pH that increased the solubility of CO₂ in the bioreactor. In addition, CO₂ removal from the biogas was likely further enhanced by an enrichment of Actinobacillus species known to be capable of CO₂ fixation. Artificial neural network (ANN) models were also used to estimate CH₄ production under different loading conditions. The ANN architecture with 10 neurons at hidden layers fit best with a mean square error of 6.06 × 10^-3 and R² of 0.99.