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Related Experiment Video

Updated: Nov 10, 2025

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Tracer-Based Cancer Metabolomic Analysis.

Jianzhou Liu1, Jing Huang1, Gary Guishan Xiao2,3

  • 1School of Pharmaceutical Science and Technology, Dalian University of Technology, Dalian, China.

Advances in Experimental Medicine and Biology
|April 1, 2021
PubMed
Summary

Cancer cells rewire metabolism to survive and spread. Stable isotope-resolved metabolomics (SIRM) uses labeled glucose tracers and advanced spectroscopy to map these metabolic pathways in the tumor microenvironment.

Keywords:
Cancer metabolomicsMSNMRSIRMStable isotope-based tracer

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Area of Science:

  • Oncology
  • Metabolomics
  • Biochemistry

Background:

  • Metabolic reprogramming is a key hallmark of cancer, enabling tumor cell proliferation, survival, and metastasis.
  • Cancer cells adapt their metabolism to harsh tumor microenvironment conditions.

Purpose of the Study:

  • To elucidate detailed mechanisms of cellular rewiring and reprogramming in the tumor microenvironment.
  • To explain the application of stable isotope tracers in cancer metabolism research.

Main Methods:

  • Stable isotope-resolved metabolomics (SIRM) utilizes stable isotope-enriched precursors to trace metabolic pathways.
  • Advanced nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS) are employed in SIRM.
  • SIRM analyzes the fate of labeled atoms from precursors to product metabolites.

Main Results:

  • SIRM enables the investigation of carbon redistribution from labeled glucose.
  • This methodology provides insights into the complex metabolic networks of cancer cells.
  • The application of SIRM in cancer metabolomic analysis is demonstrated.

Conclusions:

  • SIRM is a powerful analytical method for understanding cancer metabolism.
  • The technique elucidates the mechanisms underlying metabolic reprogramming in tumors.
  • This chapter addresses the principles, platforms, and implications of SIRM for cancer research.