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Related Concept Videos

EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

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Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
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Related Experiment Video

Updated: Nov 10, 2025

Organotypic Tissue Model Systems for Investigating Host-Pathogen Interactions In Vitro
08:41

Organotypic Tissue Model Systems for Investigating Host-Pathogen Interactions In Vitro

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Human stem cell-based models for studying host-pathogen interactions.

Enrica Pellegrino1, Maximiliano G Gutierrez1

  • 1Host-Pathogen Interactions in Tuberculosis Laboratory, The Francis Crick Institute, London, UK.

Cellular Microbiology
|April 1, 2021
PubMed
Summary
This summary is machine-generated.

Human stem cells offer advanced in vitro models for studying host-pathogen interactions, overcoming limitations of traditional cell lines. Combining stem cells with genome editing and 3D environments creates predictive models for infection research.

Keywords:
CRISPR/Cas9hostiPSCinfectionmacrophagepathogenstem cells

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Area of Science:

  • Infectious disease research
  • Stem cell biology
  • Host-pathogen interactions

Background:

  • Traditional in vitro models using cell lines and primary cells have limitations in mimicking human disease.
  • Advancements in molecular technologies necessitate more physiologically relevant in vitro systems.
  • Human stem cells, upon differentiation, can reflect in vivo cell phenotypes, revolutionizing in vitro modeling.

Purpose of the Study:

  • To discuss recent advances in using human stem cells for modeling host-pathogen interactions.
  • To highlight emerging stem cell technologies for investigating infection biology.
  • To emphasize the potential of stem cell-derived models for studying cellular responses to infection.

Main Methods:

  • Utilizing human pluripotent stem cells (hPSC) for in vitro modeling.
  • Employing genome editing technologies in conjunction with hPSC.
  • Developing engineered 3D microenvironments to enhance model relevance.

Main Results:

  • Human stem cells overcome limitations of current in vitro models for host-pathogen interaction studies.
  • Genome editing in hPSC enables detailed investigation of cellular responses to infection.
  • Integration of hPSC, 3D models, and genome editing creates highly relevant and predictive in vitro systems.

Conclusions:

  • Human stem cells are crucial for developing advanced in vitro models of infection.
  • Genome editing and 3D environments significantly enhance the predictive power of stem cell-based infection models.
  • These integrated approaches offer unprecedented potential for understanding fundamental infection biology.