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Related Concept Videos

Types of Coprecipitation01:10

Types of Coprecipitation

2.5K
Coprecipitation is the contamination of a precipitate by otherwise soluble species and occurs via different processes. In colloidal precipitates, coprecipitation occurs via surface adsorption. For instance, barium sulfate has a primary layer of adsorbed barium ions and a secondary layer of nitrate counterions. This results in contamination of the precipitate by barium nitrate.
Sometimes, ions in a crystal lattice can undergo isomorphous replacement by inclusions of similar charge and size. For...
2.5K
Precipitation and Co-precipitation01:17

Precipitation and Co-precipitation

3.1K
Precipitation and coprecipitation methods can be used to separate a mixture of ions in a solution. In qualitative inorganic analysis, ions that form sparingly soluble precipitates with the same reagent are separated based on the differences in solubility products. For example, consider the separation of Cu(II) and Fe(II) ions by precipitation as insoluble sulfides. First, copper(II) sulfide is precipitated by the addition of acidic H2S, where the dissociation of H2S is suppressed. Adding H2S...
3.1K
Precipitation Reactions03:10

Precipitation Reactions

58.8K
In a precipitation reaction, aqueous solutions of soluble salts react to give an insoluble ionic compound – the precipitate. The reaction occurs when oppositely charged ions in solution overcome their attraction for water and bind to each other, forming a precipitate that separates out from the solution. Since such reactions involve the exchange of ions between ionic compounds in aqueous solution, they are also referred to as double displacement, double replacement, exchange reactions, or...
58.8K
Precipitation Processes01:12

Precipitation Processes

2.1K
The experimental conditions in a gravimetric analysis should be optimized to maximize the particle size and purity of the obtained precipitate. Ideally, the concentration of the precipitating reagent should be low with effective stirring to maintain low relative supersaturation for the growth of large crystals. In homogeneous precipitation, the precipitant is slowly generated by a chemical reaction in the solution to avoid local reagent excesses. For example, urea decomposes gradually to...
2.1K
Precipitation Gravimetry01:03

Precipitation Gravimetry

9.9K
Precipitation gravimetry is based on converting an analyte into a sparingly soluble precipitate, which is separated by filtration and weighed. An ideal precipitate should be pure, insoluble, of known composition, and easily filtered from the reaction mixture.
In determining nickel by gravimetric analysis, a precipitant of ethanolic dimethylglyoxime is added to a hot nickel salt solution. This is quickly followed by the dropwise addition of dilute ammonia solution until precipitation occurs. A...
9.9K
Precipitation Titration: Overview01:26

Precipitation Titration: Overview

7.9K
Precipitation titration involves the reaction of a titrant and an analyte to generate an insoluble precipitate. While precipitation titration uses various precipitating agents, silver nitrate is the most common precipitating reagent; titrations involving Ag+ are called argentometric titrations. Usually, the endpoint in a precipitation titration can be detected by visual indicators.
A precipitation titration curve demonstrates the change in concentration of the titrant or analyte upon adding the...
7.9K

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Updated: Nov 10, 2025

Microbiologically Induced Calcite Precipitation Mediated by Sporosarcina pasteurii
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RNA Precipitation.

Valérie Bourguignon-Igel1,2, Yoann Abel3, Mathieu Rederstorff4

  • 1Université de Lorraine, CNRS, IMoPA, F-54000 Nancy, France.

Methods in Molecular Biology (Clifton, N.J.)
|April 1, 2021
PubMed
Summary
This summary is machine-generated.

Alcoholic precipitation is key for high-purity RNA recovery. This guide details protocols and parameters like salt, alcohol, and carriers to optimize RNA yield and purity.

Keywords:
AlcoholCarrierPrecipitationRNASalt

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Area of Science:

  • Molecular Biology
  • Biochemistry

Background:

  • RNA isolation requires high purity for downstream applications.
  • Alcoholic precipitation is a standard method for RNA recovery.

Purpose of the Study:

  • To describe the principles of alcoholic precipitation for RNA recovery.
  • To provide a standard protocol with optimization advice.
  • To discuss variations for improved efficiency and yield.

Main Methods:

  • Detailed explanation of alcoholic precipitation principles.
  • Presentation of a basic RNA precipitation protocol.
  • Discussion of critical parameters: salt, alcohol, and carrier selection.

Main Results:

  • Outlines key factors influencing RNA precipitation efficiency.
  • Highlights the impact of different reagents on RNA yield and purity.
  • Provides practical advice for successful RNA recovery.

Conclusions:

  • Optimizing alcoholic precipitation parameters is crucial for high-quality RNA.
  • Careful selection of salt, alcohol, and carriers enhances RNA recovery.
  • This chapter serves as a guide for efficient RNA isolation techniques.