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Updated: Nov 10, 2025

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
Published on: November 18, 2014
Quantitative and Qualitative Assessment of Small RNA Preparations
Virginie Marchand1, Lilia Ayadi2,3, Valérie Bourguignon-Igel2,3
1Université de Lorraine, CNRS, INSERM, IBSLOR, Nancy, France. virginie.marchand@univ-lorraine.fr.
Abstract:
Recent advances in high-throughput sequencing have shed new light on the diversity of small noncoding RNA (sncRNA) classes and their crucial roles in gene regulation and disease. One key step in sncRNA profiling consists in their quantification and assessment of their degradation extent. In this chapter, we will describe different gold standard methods used to achieve both purposes before using the sncRNAs in downstream applications.

