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Quantitative spectroscopy of single molecule interaction times.

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    We developed a new spectroscopy method to analyze interactions between indistinguishable molecules using single molecule fluorescence tracking. This technique reveals interaction times and reaction affinities from imaging data.

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    Area of Science:

    • Biophysics
    • Chemical Physics
    • Molecular Dynamics

    Background:

    • Single molecule fluorescence tracking (SMFT) offers high spatiotemporal resolution for observing molecular dynamics.
    • Quantifying interactions between indistinguishable molecules using SMFT remains challenging.

    Purpose of the Study:

    • To establish a theoretical framework for a spectroscopy of interaction times.
    • To infer molecular interactions from imaging data.

    Main Methods:

    • Development of a theoretical foundation for interaction time spectroscopy.
    • Analysis of the distribution of interaction times.
    • Validation using simulated and experimental datasets.

    Main Results:

    • A non-trivial crossover from power-law to exponential behavior in interaction time distributions was identified.
    • The exponential term's dependence on microscopic reaction affinity was demonstrated.

    Conclusions:

    • The developed spectroscopy provides quantitative insights into interactions of indistinguishable molecules.
    • This method enhances the understanding of molecular dynamics and interactions in biological systems.