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Cell Culture

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media

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Related Experiment Video

Updated: Nov 10, 2025

Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array

Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array

Published on: September 7, 2018

Single-Cell Toolkits Opening a New Era for Cell Engineering.

Sean Lee^1,2, Jireh Kim^1,2, Jong-Eun Park¹

¹Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Korea.

Molecules and Cells

|April 2, 2021

Summary

This summary is machine-generated.

Single-cell RNA sequencing (scRNA-seq) offers high-resolution transcriptome analysis. Coupling cell engineering with scRNA-seq enhances hypothesis verification for deeper biological insights.

Keywords:

CRISPR screening cell engineering lineage tracing single-cell multi-omics

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Last Updated: Nov 10, 2025

Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array

Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array

Published on: September 7, 2018

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

Micro-scale Engineering for Cell Biology

Micro-scale Engineering for Cell Biology

Published on: October 1, 2007

Area of Science:

Genomics
Molecular Biology
Cell Biology

Background:

RNA sequencing (RNA-seq) revolutionized mRNA expression analysis.
Bulk processing in early RNA-seq obscured individual cell transcriptomic patterns.
Single-cell RNA sequencing (scRNA-seq) now allows for single-cell resolution transcriptome comparison.

Purpose of the Study:

To review cell engineering toolkits.
To discuss their role in single-cell and genome-wide data collection and analysis.
To highlight their contribution to hypothesis verification in high-throughput single-cell 'omics'.

Main Methods:

Review of current cell engineering techniques.
Discussion of their integration with single-cell sequencing.
Analysis of their application in disease pathogenesis and differentiation trajectory studies.

Main Results:

Cell engineering coupled with scRNA-seq provides effective hypothesis verification strategies.
This integrated approach yields deeper insights into cellular functions and disease mechanisms.
It facilitates advanced analysis of individual cellular features.

Conclusions:

Cell engineering is crucial for advancing single-cell 'omics' research.
The integration of cell engineering and scRNA-seq enhances data collection and analysis.
This synergy is vital for understanding complex biological systems and disease dynamics.