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Peptide-based Identification of Functional Motifs and their Binding Partners
Published on: June 30, 2013
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Structure-function engineering of novel fish gelatin-derived multifunctional peptides using high-resolution
Armin Mirzapour-Kouhdasht1,2,3, Marzieh Moosavi-Nasab4,5, Chul Won Lee6
1Department of Food Science and Technology, School of Agriculture, Shiraz University, Shiraz, Iran. armin.mirzapour@shirazu.ac.ir.
Scientific Reports
|April 2, 2021
Summary
Mackerel byproducts yield multifunctional bioactive peptides with antibacterial and enzyme inhibitory properties. These fish gelatin hydrolysates show potential for food applications and health benefits.
Area of Science:
- Biochemistry
- Food Science
- Peptide Engineering
Background:
- Fish gelatin hydrolysates possess potential bioactivities not fully understood.
- Engineering multifunctional peptides from fish byproducts is an emerging area.
Purpose of the Study:
- To biologically characterize fish gelatin hydrolysates for multifunctional peptide development.
- To investigate antibacterial and enzyme inhibitory activities of mackerel-derived peptides.
Main Methods:
- Enzymatic hydrolysis of mackerel byproducts using subtilisin A and actinidin.
- In vitro assessment of antibacterial activity against food-borne pathogens.
- In vitro evaluation of angiotensin-converting enzyme (ACE) and dipeptidyl peptidase IV (DPP-IV) inhibition.
- In silico docking studies to confirm enzyme inhibition and binding affinity.
Main Results:
- Peptides exhibited broad-spectrum antibacterial activity against Gram-negative and Gram-positive bacteria.
- Significant inhibition of ACE and DPP-IV enzymes was observed, with IC50 values reported.
- In silico analysis confirmed peptide binding to ACE and DPP-IV with favorable energetics and structural stability.
Conclusions:
- Mackerel-derived bioactive peptides possess multifunctional properties, including antibacterial and enzyme inhibitory effects.
- These peptides demonstrate potential as functional ingredients in food products and nutraceuticals.
- Further research can optimize peptide production and validate their efficacy in vivo.
