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Updated: Nov 10, 2025
Measuring In Vitro ATPase Activity for Enzymatic Characterization
Published on: August 23, 2016
Determination of the stability of plasma ATP in vitro
1Department of Cardiology, The Fourth Affiliated Hospital of Zhejiang University School of Medicine Yiwu 322000, Zhejiang, P. R. China.
Background:
Adenosine 5'-triphosphate (ATP) is the most direct source of energy in organisms. Recently, it is evident that ATP plays an essential role in the immune and inflammatory systems. However, ATP is unstable when it exposed to room temperature in vitro. Therefore, our article is aim to explore the stability of ATP.
Methods And Results:
28 samples of ATP were detected. Student's t test or one-way ANOVA was used to compare multiple groups. It shows that during the storage process from day 1 to day 70, the overall levels tend to decrease.
Conclusion:
The level of ATP does not reduce at least in the first month when stored at -80°C. On the 70th day, there was a star drop, and the levels were lower than before.
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